Goat anti-Chicken IgY Antibody (HRP)

Antigen: IgY

Clonality: Polyclonal

Host: Goat

Reactivity: Chicken

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN398394

Additional Information

Immunogen: Full-length Chicken IgY (purified)

Format: Affinity-purified

Isotype: IgG

Description: Goat Anti-Chicken IgY [HRP] pAb.

Specificity: It reacts with chicken IgY heavy and light chains.

Application Details

Application Notes: Goat Anti-Chicken IgY (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 1:10,000-1:30,000. Western blot: 1:1,000-1:20,000. Other applications: user-optimized.

Concentration: 1 mg/ml

Purity: This polyclonal antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase.

Purification: Immunoaffinity chromatography

Buffer: PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal

Storage: Goat Anti-Chicken IgY (H&L) [HRP] Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Restrictions: For Research Use only