CAMP antibody

Antigen: CAMP

Clonality: Polyclonal

Host: Please enquire

Application: ELISA

Catalog no.: ABIN398435

Additional Information

Format: Affinity-purified

Isotype: IgG

Description: Adenosine 3', 5'-cyclic monophosphate (cyclic AMP, cAMP) is one of the most important intracellular secondary messengers for transduction events. cAMP is also involved in regulating neuronal, glandular, cardiovascular, immune, and other functions and actions. A number of hormones, such as ACTH, TSH, FSH, and LH, are known to activate cAMP through the action of the enzyme adenylate cyclase, which converts ATP to cAMP. There remains considerable interest in the measurement of intracellular cAMP in tissues and cell cultures, and this may help to provide an understanding of the physiology and pathology of many disease states. Due to the involvement of cAMP in amplifying the response of ligand binding, the second messenger cAMP has been largely employed to monitor the activation of GPCR to facilitate therapeutic drug discovery. Antibodies-Online Rabbit Anti-cAMP Polyclonal Antibody is developed in rabbit using 3', 5'-cyclic AMP-8-KLH as the immunogen.

Specificity: The specificity of antiserum is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding. The cross-reactivity data obtained in competitive ELISA system is as follows:

Compound	% Cross-reactivity
cAMP	100
AMP	0.0140
cGMP	0.0290
GMP	0.0223
ADP	0.0069
GDP	0.0011
ATP	<0.0001
GTP	<0.0001

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 1:4,000-1:40,000Sensitivity: Sensitivity is defined as the 90% intercept of a B/B₀ standard curve. In competitive ELISA system the sensitivity has been found to be 1-50 fmol/well.

Purification: Antiserum

Storage: Rabbit Anti-cAMP Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freeze and thaw cycles.

Research Area: Signaling

Restrictions: For Research Use only