V-ATPase Subunit A (TCIRG1) antibody

Antigen: V-ATPase Subunit A (TCIRG1)

Clonality: Polyclonal

Host: Rabbit

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN398606

Additional Information

Alternative name: V-ATPase Subunit A

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: The V-ATPase subunit A is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.Antibodies-Online Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide, purified from rabbit antiserum by immunoaffinity chromatography, and supplied as 40 ug aliquot at concentration of 0.5 mg/ml.

Specificity: Antibodies-Online Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody reacts with mouse V-ATPase subunit A. Sequence homology predicts that it will also react with human, bovine, orangutan, tobacco hornworm, chicken, fruit fly, and zebrafish V-ATPase subunit A.

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.02-0.2 µg/mlWestern blot: 1.0 µg/mlOther applications: user-optimized

Concentration: 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide

Purification: Immunoaffinity chromatography

Storage: Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody remains stable for 2-3 weeks if stored at 4°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Restrictions: For Research Use only

Images

Western blot analysis:Lane 1: EasyWestern Protein Standard (Antibodies-Online, MM0908)Lane 2: Mouse kidney tissue lysatePrimary antibody:1 ug/ml Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody (Antibodies-Online, ABIN398606)Secondary antibody:Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (Antibodies-Online, ABIN398323, 1:6,000)