5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A) antibody

Antigen: 5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A)

Synonyms: HTR2, 5-HT2A, Htr2, Htr-2, MGC124301, MGC124302, E030013E04, 5Ht-2, HTR2A, 5-HTR2A, 5HT2A, 5HTR2A, LOC100009461, Htr2a, 5HT2, DKFZp459A143

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN398705

Additional Information

Alternative name: 5HT2A Receptor

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: 5HT are receptors for neurotransmitter and peripheral signal mediator serotonin. It is also known as 5-hydroxytryptamine or 5HT. 5HT receptors mediate the effects of serotonin as the endogenous ligand and of a broad range of pharmaceutical and hallucinogenic drugs. Specifically, 5HT receptors affect the release of other neurotransmitters such as glutamate, dopamine, and GABA as well as their activities.5HT2A is a type of receptor belongs to the serotonin receptor family and is one member of G protein coupled receptor (GPCR) family. 5HT2A is involved in the contraction of tracheal smooth muscle, bronchoconstriction, and the control of aldosterone production. The receptor also happens to be necessary for the spread of the human polyoma virus called JC virus. 5HT2A is widely expressed throughout the central nervous system (CNS). It is expressed near most of the serotoninergic terminal rich areas, including neocortex and olfactory tubercle. Increased stimulation of 5HT2A receptors seems to oppose the therapeutic actions of increased stimulation of other serotonin receptors in anti-depressant and anxiolytic treatments.Antibodies-Online Rabbit Anti-5HT2A Receptor Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide derived from the third intracellular domain loop of human 5HT2A receptor (Swiss Prot: P28223).

Specificity: Antibodies-Online Rabbit Anti-5HT2A Receptor Polyclonal Antibody detects endogenous levels of 5HT2A receptor.

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.5-2 ug/mlOther applications: user-optimized

Concentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification: Immunoaffinity chromatography

Storage: Rabbit Anti-5HT2A Receptor Polyclonal Antibody should be lyophilized for storage until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Restrictions: For Research Use only

Images

Western blot analysis of mouse brain tissue lysate using 1 ug/ml Rabbit Anti-5HT2A Receptor Polyclonal Antibody (Antibodies-Online, ABIN398705)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.