Calnexin (CANX) antibody

Antigen: Calnexin (CANX)

Synonyms: CNX, P90, IP90, FLJ26570, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, DmelCG11958, CG11958, canx, MGC52927, CANX, PP90, zgc:63524, wu:fe06b12, MGC166317, MGC69400, fj49d10, MGC153946, wu:fj24b04, wu:fj49d10, zgc:153946, DKFZp459P042, LOC100283947

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN398719

Additional Information

Alternative name: Calnexin

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Calnexin is a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. As a chaperone protein, it assists in protein assembly by ensuring that only the properly folded protein is progresses along the secretory pathway. It seems to play a major role in the quality control apparatus of the ER by retaining incorrectly folded proteins.Antibodies-Online Rabbit Anti-Calnexin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 250-300 of human calnexin protein (Swiss Prot: P27824).

Specificity: Antibodies-Online Rabbit Anti-Calnexin Polyclonal Antibody detects endogenous levels of human calnexin protein. Sequence homology predicts that it will also react with mouse and rat calnexin.

Synonyms: CNX, P90, IP90, FLJ26570, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, DmelCG11958, CG11958, canx, MGC52927, CANX, PP90, zgc:63524, wu:fe06b12, MGC166317, MGC69400, fj49d10, MGC153946, wu:fj24b04, wu:fj49d10, zgc:153946, DKFZp459P042, LOC100283947

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.5-1 ug/mlOther applications: user-optimized

Concentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification: Immunoaffinity chromatography

Storage: Rabbit Anti-Calnexin Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Restrictions: For Research Use only

Images

Western blot analysis of cell lysate using 1 ug/ml Rabbit Anti-Calnexin Polyclonal Antibody (Antibodies-Online, ABIN398719)Lane 1, 3: Hela cell lysateLane 2, 4: HEK293 cell lysatePrimary antibody:Lane 1, 2: Rabbit Anti-Calnexin Polyclonal AntibodyLane 3, 4: Rabbit Anti-Calnexin Polyclonal Antibody pre-incubated with immunizing peptideThe signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.