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Tubulin, Beta, 3 (TUBB3) antibody

Antigen

Tubulin, Beta, 3 (TUBB3)
Synonyms TUBB3, TUBB4, MGC134115, MC1R, beta-4, tbb3, M(beta)3, M(beta)6, 3200002H15Rik, tubb3, MGC76202, TUBB1, tubB3, tubulin
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
Catalog no. ABIN398739
Quantity 200ug  (0.5 mg/ml, lyophilized with PBS, pH...)  (Variants)
Price 350.00 $   Plus shipping costs $35.00
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anti-Tubulin, Beta, 3 (TUBB3) antibody

anti-Tubulin, Beta, 3 (TUBB3) antibody (2)

anti-Tubulin, Beta, 3 (TUBB3) antibody (3)

Additional Information
Application Details Images Alternatives Secondary Antibodies Variants back to top

Alternative name beta-Tubulin III
Format Affinity-purified
Isotype IgG  (Matching secondary antibodies)
Description Beta-Tubulin III belongs to the tubulin family. Tubulin is the major constituent of microtubules. It forms dimers of alpha and beta chains. One mole of tubulin binds to two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. beta-Tubulin III contains a highly acidic C-terminal region which can bind cations such as calcium. It is abundant in the central and peripheral nervous systems.Antibodies-Online Rabbit Anti-beta-Tubulin III Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 150-200 of human beta-Tubulin III protein (Swiss Prot: Q13509).
Specificity Antibodies-Online Rabbit Anti-beta-Tubulin III Polyclonal Antibody detects endogenous levels of human, rat and mouse beta-tubulin III protein. Sequence homology predicts that it will also react with bovine, chicken, cynomolgus monkey. African clawed frog, and disc abalone beta-tubulin III proteins. Reactivity with other β-Tubulin had not been tested yet.

Application Details
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Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 ug/ml. Western blot: 0.5-1 ug/ml. Immunohistochemistry: 5-10 ug/ml. Flow cytometry: 1-3 ug for 1 x 10E6 cells. Other applications: user-optimized.
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Purification Immunoaffinity chromatography
Storage Rabbit Anti-beta-Tubulin III Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.
Restrictions For Research Use only