Calnexin (CANX) antibody

Antigen: Calnexin (CANX)

Synonyms: CNX, P90, IP90, FLJ26570, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, DmelCG11958, CG11958, canx, MGC52927, CANX, PP90, zgc:63524, wu:fe06b12, MGC166317, MGC69400, fj49d10, MGC153946, wu:fj24b04, wu:fj49d10, zgc:153946, DKFZp459P042, LOC100283947

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN398757

Additional Information

Alternative name: Calnexin

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Calnexin is a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may be involved in assisting protein assembly or in the retention within the endoplasmic reticulum (ER) of unassembled protein subunits. It seems to play a major role in the ER quality control apparatus via the retention of incorrectly folded proteins.Antibodies-Online Calnexin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide from within N-terminal of human calnexin protein (Swiss Prot: P27824).

Specificity: Antibodies-Online Calnexin Antibody, pAb, Rabbit detects endogenous levels of human calnexin protein.

Synonyms: CNX, P90, IP90, FLJ26570, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, DmelCG11958, CG11958, canx, MGC52927, CANX, PP90, zgc:63524, wu:fe06b12, MGC166317, MGC69400, fj49d10, MGC153946, wu:fj24b04, wu:fj49d10, zgc:153946, DKFZp459P042, LOC100283947

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.5-1 ug/mlFlow cytometry: 1-3 ug for 1 x 106 cellsOther applications: user optimized

Concentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification: Immunoaffinity chromatography

Storage: Calnexin Antibody, pAb, Rabbit should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Restrictions: For Research Use only

Images

Lane 1: Hela cell lysateLane 2: HepG2 cell lysateLane 3: HEK293 cell lysateWestern blot analysis of cell lysates using 1 ug/ml Rabbit Anti-Calnexin Polyclonal Antibody (Antibodies-Online, ABIN398757)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Flow cytometric analysis of HepG2 cells using Calnexin Antibody, pAb, Rabbit (Antibodies-Online, ABIN398757, shaded histogram) or with an isotype control antibody (Antibodies-Online, ABIN398653, open histogram), followed by R-PE conjugated anti-rabbit IgG.