Calnexin (CANX) antibody

Antigen: Calnexin (CANX)

Synonyms: CNX, P90, IP90, FLJ26570, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, DmelCG11958, CG11958, canx, MGC52927, CANX, PP90, zgc:63524, wu:fe06b12, MGC166317, MGC69400, fj49d10, MGC153946, wu:fj24b04, wu:fj49d10, zgc:153946, DKFZp459P042, LOC100283947

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Catalog no.: ABIN398769

Additional Information

Alternative name: Calnexin

Immunogen: Synthetic peptide (KLH-coupled) from carboxyl terminal of human calnexin (Swiss Prot: P27824).

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Calnexin is calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may be involved in assisting protein assembly or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the ER quality control apparatus via the retention of incorrectly folded proteins. Antibodies-Online Calnexin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide from carboxyl terminal of human calnexin protein (Swiss Prot: P27824).

Specificity: Calnexin Antibody, pAb, Rabbit detects endogenous levels of human and mouse calnexin protein. Sequence homology predicts that it will also react with rat, bovine, canine, and Sumatran orangutan calnexin.

Synonyms: CNX, P90, IP90, FLJ26570, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, DmelCG11958, CG11958, canx, MGC52927, CANX, PP90, zgc:63524, wu:fe06b12, MGC166317, MGC69400, fj49d10, MGC153946, wu:fj24b04, wu:fj49d10, zgc:153946, DKFZp459P042, LOC100283947

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml. Western blot: 0.5-1 µg/ml. Flow cytometry: 1-3 µg for 1 x 10E6 cells. Other applications: user-optimized. Predicted Band Size 90 kD. Observed Band Size 85 kD.

Concentration: 0.5 mg/ml

Purification: Immunoaffinity chromatography

Buffer: lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. Reconstitute the lyophilized powder with 80 µl of deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Storage: Calnexin Antibody, pAb, Rabbit should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Restrictions: For Research Use only

Images

Lane 1: MarkerLane 2: Hela cell lysateLane 3: HEK293 cell lysateLane 4: NIH/3T3 cell lysateLane 5: HepG2 cell lysateWestern blot analysis of cell lysates using 1 ug/ml Rabbit Anti-Calnexin Polyclonal Antibody (Antibodies-Online, ABIN398769)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Flow cytometric analysis of Ramos cells using Calnexin Antibody, pAb, Rabbit (Antibodies-Online, ABIN398769, shaded histogram) or with an isotype control antibody (Antibodies-Online, ABIN398653, open histogram), followed by R-PE conjugated anti-rabbit IgG.