Aha1 antibody

Antigen: Aha1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN398777

Additional Information

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: AHA1 is a cochaperone that stimulates HSP90 ATPase activity. It may also affect a step in the endoplasmic reticulum with regard to Golgi trafficking. AHA1 interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatitis virus glycoprotein. The protein is expressed in numerous tissues, including brain, heart, skeletal muscle, and kidney tissues and, at lower levels, liver and placental tissues.Antibodies-Online Rabbit Anti-AHA1 Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide (KLH-coupled) within residues 150-200 of human AHA1 protein (Swiss Prot: O95433).

Specificity: Antibodies-Online Rabbit Anti-AHA1 Polyclonal Antibody detects endogenous level of human AHA1 protein.

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/ml Western blot: 0.5-1 ug/mlOther applications: user optimized

Concentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification: Immunoaffinity chromatography

Storage: Rabbit Anti-AHA1 Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Research Area: Signaling, Heat Shock Proteins

Restrictions: For Research Use only

Images

Lane 1: MarkerLane 2: A549 cell lysateLane 3: K562 cell lysateLane 4: HepG2 cell lysateLane 5: rhAHA1 cell lysateWestern blot analysis of cell lysates and recombinant human AHA1 protein using 1 ug/ml Rabbit Anti-AHA1 Polyclonal Antibody (Antibodies-Online, ABIN398777)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.