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Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) antibody

Antigen

Prolyl 4-Hydroxylase, beta Polypeptide (P4HB)

Synonyms DSI, GIT, PDI, PHDB, PDIA1, PO4DB, PO4HB, PROHB, ERBA2L, P4Hbeta, Thbp, ERp59, Pdia1, PDIR, LOC100328595, p55, P4HB, DKFZp469E2031
Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Application
Alternatives ELISA, Western Blotting (WB), Flow Cytometry (FACS)
Catalog no. ABIN398915
Quantity 200ug  (0.5 mg/ml, lyophilized with PBS, pH...)  (Variants)
Price 350.00 $   Plus shipping costs $35.00
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Availability Ships within 5 to 7 Business Days

Additional Information

Alternative name PDI
Format Affinity-purified
Isotype IgG  (Matching secondary antibodies)
Description Protein Disulphide Isomerase (PDI) is a microsomal enzyme which locates in endoplasmic reticulum lumen. The enzyme catalyzes the formation and isomerization of intermolecular disulphide-bond that stabilizes three dimensional structure of many extracellular proteins. The upregulation of PDI represents an adaptive response to the accumulation of immature and denatured proteins which cause ER dysfunction in neurodegenerative disorders.Antibodies-Online Rabbit Anti-PDI Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 100-150 of human PDI (Swiss Prot: P07237).
Specificity Antibodies-Online Rabbit Anti-PDI Polyclonal Antibody detects endogenous levels of human, mouse, and rat PDI.
  • Positive Control:
  • K562, HepG2, A431, Hela, Ramos, mouse brain and rat brain lysates

    Application Details

    Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.5-1 ug/mlFlow cytometry: 1-3 ug for 1 x 106 cellsOther Applications: user-optimized
    Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
    Purification Immunoaffinity chromatography
    Storage Rabbit Anti-PDI Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.
    Research Area Cancer, Organelles
    Restrictions For Research Use only