Calreticulin (CALR) antibody

Antigen: Calreticulin (CALR)

Synonyms: RO, CRT, SSA, cC1qR, FLJ26680, crc, MGC52637, Crt, crt, Calregulin, zgc:85636, Clr, anon-EST:Liang-2.11, anon-EST:Liang-2.17, anon-EST:fe3D7, clone 2.11, clone 2.17, eth, potp, DmelCG9429, CG9429, ssa, cc1qr, MGC69541, CALR, NV19007

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN398931

Additional Information

Alternative name: Calreticulin

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Calreticulin is a multifunctional protein that serves as a major Ca2+-binding protein in the lumen of the endoplasmic reticulum. It binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of nuclear receptors. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin also serves as a recognition and clearance ligand by activating the internalization receptor LRP1 on the responding phagocyte cell surface.Antibodies-Online Rabbit Anti-Calreticulin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human Calreticulin (Swiss Prot: P27797).

Specificity: Antibodies-Online Rabbit Anti-Calreticulin Polyclonal Antibody detects endogenous levels of human Calreticulin. It is predicted to react with mouse and rat Calreticulin according to sequence homology.Positive Control: Hela and HepG2

Synonyms: RO, CRT, SSA, cC1qR, FLJ26680, crc, MGC52637, Crt, crt, Calregulin, zgc:85636, Clr, anon-EST:Liang-2.11, anon-EST:Liang-2.17, anon-EST:fe3D7, clone 2.11, clone 2.17, eth, potp, DmelCG9429, CG9429, ssa, cc1qr, MGC69541, CALR, NV19007

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.Western blot: 0.5-1 ug/mlOther Applications: user-optimized

Concentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification: Immunoaffinity chromatography.

Storage: Rabbit Anti-Calreticulin Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.

Research Area: Signaling, Organelles, Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of cell lysates using 1 ug/ml Rabbit Anti-Calreticulin Polyclonal Antibody (Antibodies-Online, ABIN398931)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.Predicted Size: 48 KDObserved Size: 48 KD