Bovine Papillomavirus Type-1 Transactivator Protein E2 (MW 48 KDa) (BPV-1 E2 Protein) antibody

Antigen: Bovine Papillomavirus Type-1 Transactivator Protein E2 (MW 48 KDa) (BPV-1 E2 Protein)

Clonality: Monoclonal (5E11)

Host: Mouse

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Catalog no.: ABIN400442

Additional Information

Characteristics: Mouse monoclonal antibody to Bovine Papillomavirus E2-protein

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 5E11

Specificity: Epitope: Mapped to amino acids 199-208 (SSTSSDFRDR)

Application Details

Application Notes: WB: 1/1000 – 1/5000; IF: 1/1000; IP: Optimal conditions for IP should be determined for each particular application. For Western blots we recommend researchers dilute the BPV-1 E2 protein antibody to a concentration of 4. 0-0. 5 µg/ml in a diluent containing 2% non-fat dry milk in TBST (Tris-Buffered Saline, Tween-20). (Background can be reduced by adding NaCl up to a concentration of 1. 5 M. )Incubate the membrane with diluted BPV-1 E2 protein antibody for 1 hour at room temperature with gentle orbital shaking. For Immunofluorescence we recommend researchers dilute BPV-1 E2 protein antibody to a concentration of 1-2 µg/ml in a diluent containing 1% BSA in PBS. Incubate with diluted BPV-1 E2 protein antibody for 1 hour at room temperature. This monoclonal antibody to BPV-1 E2 protein belongs to the IgG1 subclass, thus, we recommend using protein G-agarose for binding experiments.

Concentration: 1.00 mg/ml

Purification: IgG fraction, purified by protein A affinity chromatography

Buffer: PBS with 50% glycerol

Storage: Store at -20 to -70 ˚C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.

Research Area: Virology

Publications

Publications: Koljak, Jaﾤrving, Kurg et al.: "The basis of prostaglandin synthesis in coral: molecular cloning and expression of a cyclooxygenase from the Arctic soft coral Gersemia fruticosa." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7033-40, 2001 (PubMed).

Mannan, Boehm, Sauter et al.: "Spastin, the most commonly mutated protein in hereditary spastic paraplegia interacts with Reticulon 1 an endoplasmic reticulum protein." in: Neurogenetics, Vol. 7, Issue 2, pp. 93-103, 2006 (PubMed).