CD55 antibody

Antigen: CD55

Clonality: Monoclonal (MEM-118)

Host: Mouse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN400664

Additional Information

Characteristics: Synonyms: Complement decay-accelerating factor

Alternative name: CD55 / DAF

Swiss-Prot: P08174

Immunogen: HPB-ALL human T cell line.

Cross-Reactivity: Human, Primate

Isotype: IgM  (Matching secondary antibodies)

Clone: MEM-118

Description: Avoidance by host tissues of attack by autologous complement proteins is dependent inpart on the activities of membrane regulatory factors. One molecule involved in this controlis a 70 kD glycoprotein termed decay-accelerating factor (DAF), or CD55. Interruption byCD55 of the complement sequence at an early step in activation effectively haltsprogression of the cascade and prevents consequent cell injury. In man, CD55 is expressedon the plasma membrane of all cell types that are in intimate contact with plasmacomplement proteins. This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl oramino groups when nascent C4b and C3b are locally generated during C4 and c3activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes withtheir ability to catalyze the conversion of C2 and factor B to enzymatically active C2a andBb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertasesof the complement cascade. CD55 also acts as the receptor for echovirus 7 and related viruses(echoviruses 13, 21, 29and 33). It is attached to the membrane by a GPI-anchor.

Specificity: Recongizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widelyexpressed on hematopoietic and on many non-hematopoietic cells, it is weakly present onNK cells.

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4 containing 15 mM Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Pictures:

Restrictions: For Research Use only