Recognizes Human beta-2-Microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as soluble Human beta-2-Microglobulin. Does not cross-react with Mouse, Bovine, Rabbit, Canine (Dog) or Chicken beta-2-Microglobulin.
ELISA. Western blot: 2-4 μg/mL. Flow Cytometry: 1 μg/mL. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 10 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
PBS, pH 7.4 containing 15 mM Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Beta-2-Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin