HLA-DR-gamma (CD74) antibody

Antigen: HLA-DR-gamma (CD74)

Synonyms: DHLAG, HLADG, Ia-GAMMA, Ii, CLIP

Clonality: Monoclonal (0-N-160)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN400767

Additional Information

Characteristics: Synonyms: p33, DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DRantigens-associated invariant chain, Ia antigen-associated invariant chain

Alternative name: CD74

Swiss-Prot: P04233

Immunogen: SU-DHL-4 lymphoma cells.

Cross-Reactivity: Human, Baboon

Isotype: IgG1  (Matching secondary antibodies)

Clone: 0-N-160

Description: CD74, also known as the MHC class II associated invariant chain (Ii), is a type IItransmembrane protein which binds to the peptide binding groove of newly synthesizedMHC class II alpha/beta heterodimers and prevents their premature association withendogenous polypeptides. CD74 is produced in molar excess over MHC class II and someof this is expressed by an unknown pathway on the cell surface independent of, or inassociation with, MHC class II molecules. The half life of CD74 on the cell surface is only 3to 4 min after which it is internalized. It has been proposed that cell surface CD74 bindsMHC class II molecules which have lost their antigenic peptide leading to internalizationand reloading with antigenic peptide. The transport route of class II alpha/beta/Iicomplexes is regulated selectively by two forms of CD74 (p33 and p35) which aregenerated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (frombefore the pre-B cell stage to before the plasma cell stage), macrophages and monocytestogether, with many epithelial cells. CD74 may exist in different isoforms ranging in sizefrom 33 to 41 kDa, depending on genetic splicing.

Specificity: This antibody recognizes Human CD74 at 35kD. Recognizes B-lymphocytes and many B-cell lymphomas

Application Details

Application Notes: Western blot: 1-2 µg/ml. Flow Cytometry. Immunofluorescence. Immunohistochemistry on Frozen Sections: 2-4 µg/ml. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Purification: Purified

Buffer: 10 mM PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA and 40% Glycerol

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Pictures:

Research Area: Immunology, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only