V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody

Details for Product No. ABIN400784
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Antigen
Synonyms
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AK ... show more
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AKT, PKB/Akt, PKB/dAKT, RAC, RacPK, akt, akt1, dAKT, dAKT/dPKB, dAKT1, dAkt, dAkt/PKB, dAkt1, dPKB, dakt, dakt1, l(3)04226, l(3)89Bq, p-Akt, pAkt, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, Rac, PKBalpha, CWS6, PKB-ALPHA, PRKBA, RAC-ALPHA show less
Epitope
Ser473
(123), (75), (72), (61), (58), (56), (34), (33), (30), (29), (25), (17), (17), (16), (15), (13), (11), (9), (8), (7), (7), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chimpanzee, Human, Mouse (Murine), Rat (Rattus)
(978), (543), (503), (117), (104), (83), (65), (24), (14), (3), (3), (1), (1), (1), (1)
Host
Mouse
(839), (148), (11), (5), (4), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(31), (25), (24), (19), (14), (13), (10), (10), (10), (10), (10), (10), (10), (10), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(812), (444), (371), (164), (159), (107), (100), (75), (53), (52), (45), (35), (12), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN400784
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Immunogen Synthetic peptide corresponding to residues surrounding Ser473 of Human AKT1 protein
Clone 17F6-B11
Isotype IgG1
Specificity This phospho-specific monoclonal antibody reacts with Human and Mouse AKT pSer473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Cross-reactivity with AKT2 and AKT3 has not been determined.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Chimpanzee, Rat.
Species reactivity (tested):Human.
Characteristics Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase
Purification Protein A Chromatography
Alternative Name AKT1 / PKB
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Application Notes ELISA: 1/20,000. Immunoprecipitation. Immunoflourescence: 1/500-1/3,000. Western blot: 1/500-1/3,000 (Expect a band approximately 56 kDa in size corresponding tophosphorylated AKT protein by in the appropriate cell lysate or extract). Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections: 20 μg/mL (Nopre-treatment of sample is required).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.02 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C or below forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (2) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (Image 2)
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (3) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (Image 3)
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (4) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (Image 4)
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (5) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (Image 5)
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (6) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (Image 6)
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (7) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Ser473) antibody (Image 7)
Background publications Jones, Jakubowicz, Pitossi et al.: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991 (PubMed).

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987 (PubMed).

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001 (PubMed).

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001 (PubMed).

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