DYKDDDDK Tag antibody

Details for Product No. ABIN400788, Supplier: Log in to see
Antigen
Reactivity
Tag
233
121
65
18
18
16
9
7
4
3
2
1
1
1
Host
Mouse
194
136
34
34
17
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
40
22
19
10
10
10
9
9
8
8
8
7
7
7
6
6
6
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
334
131
126
105
87
60
50
14
11
7
7
7
4
4
4
4
4
3
3
2
1
1
1
1
1
1
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Immunogen Synthetic peptide corresponding to the DYKDDDDK epitope tag peptide DYKDDDDK(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Remarks: Residues of Glycine and Cysteine were added to the termini to facilitatecoupling.
Sequence DYKDDDDK
Clone 29E4-G7-H11
Isotype IgG2a
Characteristics Synonyms: DYKDDDDK Epitope Tag, ECS Epitope Tag, DYKDDDDK-Tag, ECS-tag, D-tag
Purification Protein A Chromatography followed by extensive dialysis against the buffer.
Alternative Name DYKDDDDK Epitope Tag
Target Type Tag
Background Sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Research Area Tags/Labels
Application Notes This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusionproteins. As such, this antibody can be used to identify fusion proteins containing theDYKDDDDK epitope. The antibody recognizes the epitope tag fused to either the amino- orcarboxy- termini of targeted proteins. The epitope tag peptide sequence was first derivedfrom the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Recommended Dilutions:
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-DYKDDDDK Tag antibody (ABIN400788) anti-DYKDDDDK Tag antibody
Western Blotting (WB) image for anti-DYKDDDDK Tag antibody (ABIN400788) anti-DYKDDDDK Tag antibody (Image 2)
Background publications Slootstra, Kuperus, Plückthun, Meloen: "Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5." in: Molecular diversity, Vol. 2, Issue 3, pp. 156-64, 1997 (PubMed).

Chubet, Brizzard: "Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells." in: BioTechniques, Vol. 20, Issue 1, pp. 136-41, 1996 (PubMed).

Robeva, Woodard, Luthin, Taylor, Linden: "Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG epitope. Development of an efficient generic protein purification procedure." in: Biochemical pharmacology, Vol. 51, Issue 4, pp. 545-55, 1996 (PubMed).

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