LY6/PLAUR Domain Containing 3 (LYPD3) (C-Term) antibody

Antigen: LY6/PLAUR Domain Containing 3 (LYPD3)

Synonyms: C4.4A, C4.4a, 2310061G07Rik, LYPD3, MGC139913

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN400952

Additional Information

Characteristics: Synonyms: C4.4A, MIG-C4, Ly6/PLAUR domain-containing protein 3, GPI-anchoredmetastasis-associated protein C4.4A homolog, Matrigel-induced gene C4 protein

Alternative name: LYPD3

Swiss-Prot: O95274

Immunogen: Synthetic peptide KLH conjugated. LYPD3 antibody was raised against C-terminal domain of Human LYPD3

Cross-Reactivity: Human

Isotype: IgG  (Matching secondary antibodies)

Description: C4. 4A is a structural homolog of the urokinase-type plasminogen activator receptor (uPAR). It was originally identified as a metastasis-associated membrane protein in malignanturothelium. In literature IHC studies, staining has been observed in suprabasalkeratinocytes of chronic wounds and the amniotic membrane of placenta. ISH analysisdemonstrated expression in urothelium of the ureter. C4. 4A expression has also been found to be upregulated in breast tumors when compareto normal breast tissue.

Specificity: This antibody recognizes GPI-anchored Metastasis-associated Protein Homolog (C4. 4A)(LYPD3). Species Reactivity: Tested: Human.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 15 µg/ml. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen. The stained slides were evaluated by apathologist to confirm staining specificity. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.6 mg/ml

Purification: Aff - Purified

Buffer: PBS containing 0.09% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only