Notch 2 (NOTCH2) (N-Term), (cleaved) antibody

Details for Product No. ABIN400960
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Synonyms AGS2, HJCYS, hN2, AI853703, N2, notch6, sb:cb884
N-Term, cleaved
(15), (13), (13), (6), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(126), (64), (54), (7), (2), (2), (2), (2), (2), (1), (1), (1)
(109), (23), (10), (8), (3), (2)
(7), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(97), (86), (35), (30), (22), (10), (10), (6), (5), (4), (2), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 0.2 mL
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Catalog No. ABIN400960
489.50 $
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Immunogen Synthetic peptide corresponding to amino acid residues of human Notch 2 located near the N-terminal sequence of the cleaved N intracellular domain (NICD)
Isotype IgG
Specificity This antiserum is directed against NOTCH 2.
Cross-Reactivity (Details) Species reactivity (expected):Rat and Mouse: Only one amino acid difference (conservative) occurs.
Species reactivity (tested):Human.
Purification Serum
Alternative Name NOTCH2
Background Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.Synonyms: Neurogenic locus notch homolog protein 2, Notch 2, hN2
Gene ID 4853
NCBI Accession NP_001186930
UniProt Q04721
Application Notes A strong response was detected by ELISA against the immunizing peptide. This productwas assayed against the peptide immunogen in a standard capture ELISA using Peroxidaseconjugated anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (code #ABTS-100) as a substrate for30 minutes at room temperature. A working dilution of 1/30,000 to 1/90,000 is suggestedfor this product. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/400-1/2,000. Immunohistochemsitry on Paraffin Sections: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 70 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative without stabilizers.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
anti-Notch 2 (NOTCH2) (N-Term), (cleaved) antibody anti-Notch 2 (NOTCH2) (N-Term), (cleaved) antibody
Background publications Gridley: "Notch signaling and inherited disease syndromes." in: Human molecular genetics, Vol. 12 Spec No 1, pp. R9-13, 2003 (PubMed).

Bertrand, Eckfeldt, Lysholm et al.: "Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells." in: Leukemia, Vol. 14, Issue 12, pp. 2095-102, 2001 (PubMed).

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