Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 56-239), (His6) antibody

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Synonyms smac, smac3, diablo-s, 0610041G12Rik, 1700006L01Rik, AU040403, Smac, DFNA64, SMAC, DBO, XSmac
AA 56-239, His6
(41), (14), (13), (13), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
(152), (58), (37), (4), (4), (3), (2), (1), (1)
(134), (29), (8), (1)
(1), (1)
Western Blotting (WB)
(161), (65), (58), (36), (25), (11), (8), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Pubmed 7 references available
Quantity 0.1 mL
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Immunogen Recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239)
Isotype IgG
Specificity This antiserum is directed against Smac/DIABLO and is useful in determining its presence in various assays.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Serum
Alternative Name DIABLO / SMAC (DIABLO Antibody Abstract)
Background Apoptosis is a conserved cell suicide program essential for the development and homeostasis of multicellular organisms. Abnormal inhibition of apoptosis is a hallmark of cancer and autoimmune diseases, whereas excessive cell death is found in neurodegenerative disorders such as Alzheimers disease. Executioners of the apoptotic program are cysteine proteases termed caspases that exist as inactive zymogens in living cells and are activated during apoptosis. Active caspases cleave key intracellular protein substrates, resulting in the characteristic morphological changes associated with apoptosis. The release of cytochrome c from the mitochondria triggers the oligomerization of Apaf-1 in an ATP/dATP-dependent manner and induces the autoactivation of caspase-9. Active caspase-9 in turn activates downstream effector caspases including caspase -3, -6 and -7. Given the potentially irreversible cascade triggered by the activation of initiator caspases, it is crucial that activation of caspases be tightly regulated. A number of cellular proteins have been shown to modulate caspase activation and activity. These include members of the Inhibitor of the Apoptosis Protein (IAP) family, such as XIAP, c-IAP-1 and c-IAP-2, which block cell death by inhibiting the activity of the effector caspases -3 and -7 and the initiator caspase-9. Smac/DIABLO (Second Mitochondria-derived Activator of Caspase) is a mitochondrial protein of 27 kDa that is released from mitochondria during apoptosis. Smac/DIABLO promotes caspase activation through binding and neutralizing IAPs.Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 56616
NCBI Accession NP_063940
UniProt Q9NR28
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western blot: 1: 1000 to 1: 2000 dilution. A 27 kDa band corresponding to humanSmac/DIABLO protein is detected. Detection occurs using HRP Donkey anti-Rabbit IgG andECL. As little as 10 ng of recombinant protein can be detected by ECL. Positive control: Lysates from human HeLa and LNCaP cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C for longer.
Supplier Images
Image no. 1 for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 56-239), (His6) antibody (ABIN400971) anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 56-239), (His6) antibody
Background publications Verhagen, Coulson, Vaux: "Inhibitor of apoptosis proteins and their relatives: IAPs and other BIRPs." in: Genome biology, Vol. 2, Issue 7, pp. REVIEWS3009, 2001 (PubMed).

Wu, Chai, Suber et al.: "Structural basis of IAP recognition by Smac/DIABLO." in: Nature, Vol. 408, Issue 6815, pp. 1008-12, 2001 (PubMed).

Liu, Sun, Olejniczak et al.: "Structural basis for binding of Smac/DIABLO to the XIAP BIR3 domain." in: Nature, Vol. 408, Issue 6815, pp. 1004-8, 2001 (PubMed).

Chai, Du, Wu et al.: "Structural and biochemical basis of apoptotic activation by Smac/DIABLO." in: Nature, Vol. 406, Issue 6798, pp. 855-62, 2000 (PubMed).

Srinivasula, Datta, Fan et al.: "Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36152-7, 2000 (PubMed).

Verhagen, Ekert, Pakusch et al.: "Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins." in: Cell, Vol. 102, Issue 1, pp. 43-53, 2000 (PubMed).

Du, Fang, Li et al.: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000 (PubMed).

Catalog No. ABIN400971
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