Did you know that you can buy products from over 140 different suppliers from us?

Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 56-239), (His6) antibody

Details for Product No. ABIN400971
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms Smac, DFNA64, SMAC, DBO, XSmac, smac, smac3, diablo-s
Epitope
AA 56-239, His6
(27), (8), (7), (3), (3), (3), (3), (2), (2), (1), (1)
Reactivity
Human
(124), (56), (47), (14), (14), (13), (13), (2), (1)
Host
Rabbit
(110), (20), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(121), (42), (39), (35), (15), (10), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1)
Pubmed 7 references available
Quantity 0.1 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN400971
390.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239)
Isotype IgG
Specificity This antiserum is directed against Smac/DIABLO and is useful in determining its presence in various assays.
Purification Serum
Alternative Name DIABLO / SMAC
Background Apoptosis is a conserved cell suicide program essential for the development and homeostasis of multicellular organisms. Abnormal inhibition of apoptosis is a hallmark of cancer and autoimmune diseases, whereas excessive cell death is found in neurodegenerative disorders such as Alzheimers disease. Executioners of the apoptotic program are cysteine proteases termed caspases that exist as inactive zymogens in living cells and are activated during apoptosis. Active caspases cleave key intracellular protein substrates, resulting in the characteristic morphological changes associated with apoptosis. The release of cytochrome c from the mitochondria triggers the oligomerization of Apaf-1 in an ATP/dATP-dependent manner and induces the autoactivation of caspase-9. Active caspase-9 in turn activates downstream effector caspases including caspase -3, -6 and -7. Given the potentially irreversible cascade triggered by the activation of initiator caspases, it is crucial that activation of caspases be tightly regulated. A number of cellular proteins have been shown to modulate caspase activation and activity. These include members of the Inhibitor of the Apoptosis Protein (IAP) family, such as XIAP, c-IAP-1 and c-IAP-2, which block cell death by inhibiting the activity of the effector caspases -3 and -7 and the initiator caspase-9. Smac/DIABLO (Second Mitochondria-derived Activator of Caspase) is a mitochondrial protein of 27 kDa that is released from mitochondria during apoptosis. Smac/DIABLO promotes caspase activation through binding and neutralizing IAPs.
Alternate names: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 56616
NCBI Accession NP_063940.1
UniProt Q9NR28
Application Notes Western blot: 1: 1000 to 1: 2000 dilution. A 27 kDa band corresponding to humanSmac/DIABLO protein is detected. Detection occurs using HRP Donkey anti-Rabbit IgG andECL. As little as 10 ng of recombinant protein can be detected by ECL. Positive control: Lysates from human HeLa and LNCaP cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C for longer.
Expiry Date 12 months
Supplier Images
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 56-239), (His6) antibody anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 56-239), (His6) antibody
Background publications Du, Fang, Li et al.: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000 (PubMed).

Verhagen, Ekert, Pakusch et al.: "Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins." in: Cell, Vol. 102, Issue 1, pp. 43-53, 2000 (PubMed).

Srinivasula, Datta, Fan et al.: "Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36152-7, 2000 (PubMed).

Chai, Du, Wu et al.: "Structural and biochemical basis of apoptotic activation by Smac/DIABLO." in: Nature, Vol. 406, Issue 6798, pp. 855-62, 2000 (PubMed).

Liu, Sun, Olejniczak et al.: "Structural basis for binding of Smac/DIABLO to the XIAP BIR3 domain." in: Nature, Vol. 408, Issue 6815, pp. 1004-8, 2001 (PubMed).

Wu, Chai, Suber et al.: "Structural basis of IAP recognition by Smac/DIABLO." in: Nature, Vol. 408, Issue 6815, pp. 1008-12, 2001 (PubMed).

Verhagen, Coulson, Vaux: "Inhibitor of apoptosis proteins and their relatives: IAPs and other BIRPs." in: Genome biology, Vol. 2, Issue 7, pp. REVIEWS3009, 2001 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN400971
390.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
Did you look for something else?
back to top