Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 305-323) antibody

Antigen: Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4)

Synonyms: CaMK-GR, MGC36771, CAMK4, CaMKIV, AI666733, CaMKIV/Gr, D18Bwg0362e, A430110E23Rik, Ccdpk, RATCCDPK, camk4-A, wu:fj42a03, zgc:110275

Epitope: AA 305-323

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine)

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN400973

Additional Information

Characteristics: Synonyms: CaMK IV, CAM kinase-GR, Calcium/calmodulin-dependent protein kinase type IV

Alternative name: CAMK4

Swiss-Prot: Q16566

Immunogen: Synthetic peptide corresponding to amino acids 305-323 of Human CaM Kinase IV protein

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chimpanzee, Chicken, Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG  (Matching secondary antibodies)

Description: CaM Kinase IV (also known as CAM kinase-GR, CAMK4 and CaMK IV) is a calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signalingcascade. This kinase may be involved in the transcriptional regulation of microtubuledynamics. In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D andSTMN1/OP18. CaMK IV may also be involved in spermatogenesis and may play a role in theconsolidation/ retention of hippocampusdependent long-term memory. CaMK IV must bephosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2. Inaddition autophosphorylation of the N-terminus is equired for full activation. Autophosphorylation of Ser-336 allows the kinase to switch to aCa(2+)/calmodulin-independent state. Most likely the kinase is inactivated by the serine/threonine protein phosphatase 2A. CaMK IV is a monomer that is located within thecytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei. Inspermatids CaMK IV is associated with chromatin and the nuclear matrix. CaMK IV is alsospecifically expressed in epithelial ovarian cancer tissue.

Specificity: This antibody reacts to CAMK4. Species Reactivity: Tested: Human, Mouse, Rat, Canine, Bovine, Chimpanzee. Expected from sequence similarity: Zebrafish, Chicken, Frog.

Application Details

Application Notes: Immunohistochemistry. ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500 - 1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 70 mg/ml by Refractometry

Purification: Serum

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide as preservativeReconstitution: Restore with 0.1 ml of deionized water (or equivalent).

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one monthor (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only