Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 601-1115) antibody

Details for Product No. ABIN400976
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Antigen
Synonyms EIF2AK3, AI427929, Pek, Perk, PEK, PERK, WRS
Epitope
AA 601-1115
(20), (13), (12), (11), (6), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(98), (48), (43), (12), (12), (12)
Host
Rabbit
(92), (11), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(63), (57), (47), (20), (18), (10), (8), (5), (4), (2), (1)
Pubmed 8 references available
Quantity 0.1 mL
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Catalog No. ABIN400976
390.50 $
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Immunogen Recombinant fusion protein from amino acids 601-1115 of Mouse deltaN PERK
Isotype IgG
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Serum
Alternative Name EIF2AK3
Background The PKR-like endoplasmic reticulum kinase (PERK, also know as Eukaryotic translation initiation factor 2-alpha kinase 3) is a type I transmembrane protein localized to the endoplasmic reticulum (ER). PERK consists of an N-terminal ER luminal domain, a membrane-spanning region, and a cytosolic C-terminal serine/threonine kinase domain (1). The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells (2). PERK activation occurs upon accumulation of misfolded proteins and the ER lumen, which triggers GRP78 dissociation from PERK thereby allowing PERK dimerization and autophosphorylation (3, 4). PERK phosphorylates two established targets: the eukaryotic translation initiation factor 2 alpha (eIF2alpha, (1)) and the Nrf2 transcription factor (5). Phosphorylation of eIF2alpha results in attenuation of translation initiation (6). The translational block also contributes to cell cycle arrest due to loss of the G1 regulatory protein, cyclin D1 (7). PERK-dependent phosphorylation of Nrf2 promotes transcription of phase II detoxifying enzymes which is critically important for elimination of intracellular reactive oxygen species (8). Thus, while inhibiting new protein synthesis and thereby decreasing the ER protein load PERK simultaneously induces expression of genes that help restore cellular redox homeostasis and promote survival.Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 3, HsPEK, PEK, PERK, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase
Gene ID 10090
UniProt Q9Z2B5
Application Notes ELISA: 1/4,000-1/20,0000. Western blot: 1/500-1/3000. Expect bands of approx. 150 kDa in the appropriate cell lysate orextract. Immunoprecipitaion: 10-30 μL. Immunoflourescence. Immunohistochemistry on Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 601-1115) antibody anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 601-1115) antibody
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 601-1115) antibody (2) anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 601-1115) antibody (Image 2)
Background publications Harding, Zhang, Ron: "Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase." in: Nature, Vol. 397, Issue 6716, pp. 271-4, 1999 (PubMed).

Kimball: "Eukaryotic initiation factor eIF2." in: The international journal of biochemistry & cell biology, Vol. 31, Issue 1, pp. 25-9, 1999 (PubMed).

Brewer, Hendershot, Sherr et al.: "Mammalian unfolded protein response inhibits cyclin D1 translation and cell-cycle progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 15, pp. 8505-10, 1999 (PubMed).

Liu, Schröder, Kaufman: "Ligand-independent dimerization activates the stress response kinases IRE1 and PERK in the lumen of the endoplasmic reticulum." in: The Journal of biological chemistry, Vol. 275, Issue 32, pp. 24881-5, 2000 (PubMed).

Bertolotti, Zhang, Hendershot et al.: "Dynamic interaction of BiP and ER stress transducers in the unfolded-protein response." in: Nature cell biology, Vol. 2, Issue 6, pp. 326-32, 2000 (PubMed).

Ma, Vattem, Wek: "Dimerization and release of molecular chaperone inhibition facilitate activation of eukaryotic initiation factor-2 kinase in response to endoplasmic reticulum stress." in: The Journal of biological chemistry, Vol. 277, Issue 21, pp. 18728-35, 2002 (PubMed).

Cullinan, Zhang, Hannink et al.: "Nrf2 is a direct PERK substrate and effector of PERK-dependent cell survival." in: Molecular and cellular biology, Vol. 23, Issue 20, pp. 7198-209, 2003 (PubMed).

Lee, Surh: "Nrf2 as a novel molecular target for chemoprevention." in: Cancer letters, Vol. 224, Issue 2, pp. 171-84, 2005 (PubMed).

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