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IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term)

Details for Product anti-IRAK1 Antibody No. ABIN401038, Supplier: Login to see
Antigen
  • IRAK1
  • IRAK
  • pelle
  • AA408924
  • IRAK-1
  • IRAK1-S
  • Il1rak
  • Plpk
  • mPLK
  • RGD1563841
Alternatives
anti-Human IRAK1 antibody for Immunofluorescence
Epitope
C-Term
37
35
22
17
15
14
14
13
13
13
13
9
8
7
6
5
5
5
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
226
111
78
Host
Rabbit
215
41
2
Clonality
Polyclonal
Conjugate
This IRAK1 antibody is un-conjugated
11
8
7
5
4
4
4
4
4
4
4
4
3
3
3
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
179
108
52
45
45
40
29
19
16
9
9
3
2
Supplier
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Immunogen Synthetic peptide corresponding to a region near the carboxy terminus of Human IRAK protein.
Isotype IgG
Specificity This IgG fraction antibody is directed against Human IRAK protein. A BLAST analysis was used to suggest cross reactivity with IRAK protein from Human, Mouse and Rat based on 100 % homology with the immunizing sequence. No Cross reactivity occurs against IRAK-2. Reactivity against homologues from other sources is not known.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.
Purification DEAE Chromatography
Alternative Name IRAK1 (IRAK1 Antibody Abstract)
Background IRAK (Interleukin-1 receptor-associated kinase 1). Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).Synonyms: IRAK, IRAK-1, Interleukin-1 receptor-associated kinase 1
Gene ID 3654
NCBI Accession NP_001020413
UniProt P51617
Research Area Cytokines, Receptors, Inflammation
Pathways NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway
Application Notes ELISA: 1/5000 - 1/20000. Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/500. Immunoprecipitation: 2-4 μg per sample.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term) (ABIN401038) anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody
 image for anti-IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term) (ABIN401038) anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody (Image 2)
Background publications Yao, Li, Shi: "Optically active supramolecular complexes of water-soluble achiral polythiophenes and folic acid: spectroscopic studies and sensing applications." in: Langmuir : the ACS journal of surfaces and colloids, Vol. 24, Issue 22, pp. 12829-35, 2008 (PubMed).

Huang, Gao, Li et al.: "Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 12829-32, 1998 (PubMed).

Robinson, Shibuya, Mui et al.: "IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NFkappaB." in: Immunity, Vol. 7, Issue 4, pp. 571-81, 1997 (PubMed).

Trofimova, Sprenkle, Green et al.: "Developmental and tissue-specific expression of mouse pelle-like protein kinase." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17609-12, 1996 (PubMed).

Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996 (PubMed).