Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody

Details for Product No. ABIN401038
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms IRAK1, IRAK, pelle, AA408924, IRAK-1, IRAK1-S, Il1rak, Plpk, mPLK, RGD1563841
Epitope
C-Term
(33), (33), (29), (24), (14), (8), (8), (8), (7), (6), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(208), (100), (78), (36), (24), (24), (12)
Host
Rabbit
(198), (40), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(12), (10), (10), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(159), (115), (58), (50), (40), (36), (22), (21), (19), (7), (3), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN401038
368.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptide corresponding to a region near the carboxy terminus of Human IRAK protein.
Isotype IgG
Specificity This IgG fraction antibody is directed against Human IRAK protein. A BLAST analysis was used to suggest cross reactivity with IRAK protein from Human, Mouse and Rat based on 100% homology with the immunizing sequence. No Cross reactivity occurs against IRAK-2. Reactivity against homologues from other
Purification DEAE Chromatography
Alternative Name IRAK1
Background IRAK (Interleukin-1 receptor-associated kinase 1). Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).
Alternate names: IRAK, IRAK-1, Interleukin-1 receptor-associated kinase 1
Gene ID 3654
NCBI Accession NP_001020413
UniProt P51617
Research Area Cytokines, Receptors, Inflammation
Application Notes ELISA: 1/5000 - 1/20000. Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/500. Immunoprecipitation: 2-4 µg per sample. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody
anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody (2) anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody (Image 2)
Background publications Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996 (PubMed).

Trofimova, Sprenkle, Green et al.: "Developmental and tissue-specific expression of mouse pelle-like protein kinase." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17609-12, 1996 (PubMed).

Robinson, Shibuya, Mui et al.: "IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NFkappaB." in: Immunity, Vol. 7, Issue 4, pp. 571-81, 1997 (PubMed).

Huang, Gao, Li et al.: "Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 12829-32, 1998 (PubMed).

Yao, Li, Shi: "Optically active supramolecular complexes of water-soluble achiral polythiophenes and folic acid: spectroscopic studies and sensing applications." in: Langmuir : the ACS journal of surfaces and colloids, Vol. 24, Issue 22, pp. 12829-35, 2008 (PubMed).

Validation Images
Did you look for something else?
back to top