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CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator)

Details for Product anti-CFLAR Antibody No. ABIN401040, Supplier: Login to see
Antigen
  • Clarp1
  • cflar
  • CFLAR
  • FLIP
  • CASH
  • CASP8AP1
  • CLARP
  • Casper
  • FLAME
  • FLAME-1
  • FLAME1
  • I-FLICE
  • MRIT
  • c-FLIP
  • c-FLIPL
  • c-FLIPR
  • c-FLIPS
  • 2310024N18Rik
  • A430105C05Rik
  • Cash
  • ENSMUSG00000072980
  • Flip
  • Gm9845
  • c-Flip
Alternatives
anti-Human CFLAR antibody for Immunofluorescence
Reactivity
Human, Mouse (Murine), Rat (Rattus)
176
78
56
2
2
2
2
2
1
Host
Rabbit
146
47
3
Clonality
Polyclonal
Conjugate
This CFLAR antibody is un-conjugated
6
3
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
155
59
38
36
30
23
22
12
11
8
3
1
1
1
Supplier
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Immunogen Synthetic peptide corresponding to amino acids near the carboxyl terminus of the Human FLIPalpha/FLIPL protein . Genename: CFLAR
Isotype IgG
Specificity This antibody is directed against Human FLIP-alpha protein and is specific for the a splice variant.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Purification DEAE Ion Exchange Chromatography.
Alternative Name CFLAR / Casper / I-FLICE (CFLAR Antibody Abstract)
Background Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP
Gene ID 8837
NCBI Accession NP_001120655
UniProt O15519
Pathways Apoptosis
Application Notes Western Blot: 0.5-1 μg/mL. Detects a band at approximately 55 kDa. recommended Positive Control: HeLa whole cell lysate. Immunohistochemistry: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.02 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (ABIN401040) anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) antibody
 image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (ABIN401040) anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) antibody (Image 2)
Background publications Irmler, Thome, Hahne et al.: "Inhibition of death receptor signals by cellular FLIP." in: Nature, Vol. 388, Issue 6638, pp. 190-5, 1997 (PubMed).

Hu, Vincenz, Ni et al.: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997 (PubMed).

Shu, Halpin, Goeddel: "Casper is a FADD- and caspase-related inducer of apoptosis." in: Immunity, Vol. 6, Issue 6, pp. 751-63, 1997 (PubMed).

Thome, Schneider, Hofmann et al.: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997 (PubMed).