GPC1 antibody (Internal Region)

Catalog No. ABIN401058

Product Details anti-GPC1 Antibody

Target: GPC1 (Glypican 1 (GPC1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog, Macaque, Opossum

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPC1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific for Glypican-1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Macaque, Canine, Cattle, Opossum.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide corresponding to an internal region of Human Glypican-1 protein.

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 2,500 - 1: 3000. Western Blot: 1: 800 - 1: 1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20° C prior to opening. Dilute only prior to immediate use. Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for GPC1

Target: GPC1 (Glypican 1 (GPC1))

Alternative Name: Glypican-1 / GPC1 (GPC1 Products)

Synonyms: gpc1 antibody, zgc:63947 antibody, GPC1 antibody, Glypican-1 antibody, glypican antibody, AI462976 antibody, HSPG M12 antibody, HSPGM12 antibody, glypican 1b antibody, glypican 1 antibody, gpc1b antibody, GPC1 antibody, gpc1 antibody, Gpc1 antibody

Background: Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary from 17 % to 63 % identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican-1 has been shown to interact with SLIT2.

Gene ID: 2817

NCBI Accession: NP_002072

UniProt: P35052

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation