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IL-21 antibody (AA 24-152)

IL21 Reactivity: Cow WB, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN401062
  • Target See all IL-21 (IL21) Antibodies
    IL-21 (IL21) (Interleukin 21 (IL21))
    Binding Specificity
    • 15
    • 11
    • 7
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 24-152
    Reactivity
    • 81
    • 29
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Cow
    Host
    • 68
    • 16
    • 5
    • 4
    • 1
    Rabbit
    Clonality
    • 80
    • 14
    Polyclonal
    Conjugate
    • 50
    • 12
    • 6
    • 6
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL-21 antibody is un-conjugated
    Application
    • 69
    • 57
    • 13
    • 12
    • 9
    • 7
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificity
    This antibody reacts to Interleukin-21.
    Cross-Reactivity (Details)
    Species reactivity (expected):Porcine, Horse, Human, Canine, Macaque, Platypus and Syrian hamster, Mouse, Rat, Chicken.
    Species reactivity (tested):Bovine.
    Purification
    Affinity chromatography on Protein A
    Immunogen
    Recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein
    Isotype
    IgG
    Top Product
    Discover our top product IL21 Primary Antibody
  • Application Notes
    ELISA: 1/6,000. Western Blot: 1 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL by UV absorbance at 280 nm
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    IL-21 (IL21) (Interleukin 21 (IL21))
    Alternative Name
    Interleukin-21 / IL21 (IL21 Products)
    Synonyms
    IL-21 antibody, Za11 antibody, interleukin 21 antibody, IL21 antibody, Il21 antibody
    Background
    Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common ?-chain with IL-2, IL-4, IL-7, IL-9, and IL-15 proteins but, in addition, binds to a unique IL-21Ra chain which triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Bovine and human IL-21 have pleiotropic functions and are mainly produced by activated T-cells in response to antigenic stimulation, but target a broad range of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells' (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells, with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78-81 % and 58-67 % homologous to the predicted human and murine IL-21 amino acid sequences, respectively. In one study, recombinant bovine IL-21 strongly induced NK cell proliferation using a human NK cell-line, NK0, and enhanced the lymphokine activated killer (LAK) activity of bovine peripheral blood mononuclear cells. In another by the same authors, recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MATSynonyms: IL-21, Za11
    Gene ID
    378475
    NCBI Accession
    NP_942129
    UniProt
    Q76LU5
    Pathways
    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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