HA-Tag antibody (HRP)

Details for Product No. ABIN401239
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Antigen
Reactivity
Tag
(183), (22), (9), (7), (5), (3), (2), (2), (1)
Host
Rabbit
(98), (93), (19), (7), (2), (1)
Clonality
Polyclonal
Conjugate
HRP
(21), (13), (10), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(158), (71), (60), (50), (30), (27), (18), (15), (10), (9), (6), (5), (4), (4), (3), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN401239
434.50 $
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Immunogen Purified from whole Rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from Hemagglutinin Influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling
Isotype IgG
Specificity This antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. In Western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
Purification Affinity Chromatography
Alternative Name HA Epitope Tag (YPYDVPDYA)
Target Type Tag
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti- epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Application Notes Western Blot: 1/1,000 - 1/5,000. ELISA: 1/15,000. Immunohistochemistry: 1/500 - 1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 0.1 mL of deionized water (or equivalent).
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 0.01% (w/v) Gentamicin Sulfate 10 mg/ml BSA
Preservative Gentamicin sulfate
Handling Advice Avoid repeated freezing and thawing. Do Not Add Sodium Azide
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Background publications Field, Nikawa, Broek et al.: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).

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