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PRKDC antibody (Thr2609)

PRKDC Reactivity: Human, Mouse, Rat, Chicken, Dog, Horse, Chimpanzee WB, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN401263
  • Target See all PRKDC Antibodies
    PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))
    Binding Specificity
    • 26
    • 13
    • 12
    • 7
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Thr2609
    Reactivity
    • 162
    • 91
    • 49
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    Human, Mouse, Rat, Chicken, Dog, Horse, Chimpanzee
    Host
    • 154
    • 21
    • 3
    • 2
    Rabbit
    Clonality
    • 149
    • 31
    Polyclonal
    Conjugate
    • 96
    • 11
    • 11
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    This PRKDC antibody is un-conjugated
    Application
    • 85
    • 70
    • 58
    • 36
    • 34
    • 28
    • 27
    • 24
    • 14
    • 13
    • 8
    • 7
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificity
    This antibody reacts with phosphorylated pThr2609 of DNA PKcs. Reactivity with non-phosphorylated Human DNA PKcs is minimal. Cross-reactivity with DNA PKcs homologues from other sources has not been determined.
    Cross-Reactivity (Details)
    Species reactivity (expected):Chimpanzee (100 %), Mouse, Rat, Dog, Chicken.
    Species reactivity (tested):Human.
    Purification
    Immunoaffinity Chromatography. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
    Immunogen
    Synthetic peptide corresponding to amino acids surrounding Thr 2609 of Human DNA PKcs
    Isotype
    IgG
    Top Product
    Discover our top product PRKDC Primary Antibody
  • Application Notes
    ELISA: 1/300,000. Western blot: 1/500-1/2,000. Expect a band approx. 460 kDa in size corresponding to DNA PKcsin the appropriate cell lysate or extract. Best western blotting results are seen when Immunoprecipitation is performed prior todetection. This antibody detects an inducible signal at the correct height after DNAdamage. However, the antibody may also detect an IR-inducible signal in cells lacking DNAPKcs (MO59J cells) at the same size as DNA PKcs if western blotting is performed directly. We believe that this additional band is 53BP1 that runs at the same size as DNA PKcs and isalso phosphorylated in an IR-dependent manner. Similar results were seen with anotherphospho DNA PKcs antibody indicating that this result is general to antibodies to thisphospho site, rather than specific to this antibody.
    Restrictions
    For Research Use only
  • Concentration
    0.93 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))
    Alternative Name
    DNA-PKcs / PRKDC / XRCC7 (PRKDC Products)
    Synonyms
    DNA-PKcs antibody, DNAPK antibody, DNPK1 antibody, HYRC antibody, HYRC1 antibody, XRCC7 antibody, p350 antibody, DNA-PKCS antibody, dna-pkcs antibody, dnapk antibody, dnpk1 antibody, hyrc antibody, hyrc1 antibody, prkdc-A antibody, xrcc7 antibody, PRKDC antibody, AI326420 antibody, AU019811 antibody, DNAPDcs antibody, DOXNPH antibody, dxnph antibody, p460 antibody, scid antibody, slip antibody, protein kinase, DNA-activated, catalytic polypeptide antibody, protein kinase, DNA-activated, catalytic polypeptide L homeolog antibody, DNA-dependent protein kinase catalytic subunit antibody, protein kinase, DNA activated, catalytic polypeptide antibody, PRKDC antibody, prkdc.L antibody, LOC100633457 antibody, Prkdc antibody
    Background
    DNA dependent Protein Kinase (also called DNAPK, DNPK1, HYRC1, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC7 and p460) consists of the 460 kDa DNA PKcs and a heterodimeric regulatory complex comprised of p70 Ku and p80 Ku (Ku autoantigen). DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T antigen. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide. Alternative splicing can occur for this protein to produce at least two isoforms.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460
    Molecular Weight
    469.1 kDa
    Gene ID
    5591
    NCBI Accession
    NP_001075109
    UniProt
    P78527
    Pathways
    DNA Damage Repair, Production of Molecular Mediator of Immune Response
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