FANCA antibody (AA 995-1009)

Catalog No. ABIN401266

Product Details anti-FANCA Antibody

Target: FANCA (Fanconi Anemia Group A Protein (FANCA))

Binding Specificity: AA 995-1009

Reactivity: Human, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FANCA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against human FANCA protein.

Cross-Reactivity (Details): Species reactivity (tested):Human, Chimpanzee.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 995-1009 of human FANCA protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 15,000 - 1: 60,000. Western blot: 1: 500 - 1: 3,000 (Expect a main band at approximately 163 kDa in sizecorresponding to FANCA by western blotting in the appropriate cell lysate or extract orhuman tissue).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.45 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for FANCA

Target: FANCA (Fanconi Anemia Group A Protein (FANCA))

Alternative Name: FANCA (FANCA Products)

Synonyms: FA antibody, FA-H antibody, FA1 antibody, FAA antibody, FACA antibody, FAH antibody, FANCH antibody, AW208693 antibody, Fanconi anemia complementation group A antibody, Fanconi anemia, complementation group A antibody, FANCA antibody, Fanca antibody

Background: FANCA (also called Protein FACA or Fanconi anemia group A protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCA may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, and FANCL. The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity, they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. Variant 1 (isoform a) encodes the longest transcript. Variant 2 (isoform b) contains an alternate exon, which results in an early stop codon, compared to variant 1. Isoform b has a shorter C-terminus when compared to isoform a. Mutations in this gene are the most common cause of Fanconi anemia.Synonyms: FAA, FACA, FANCH, Fanconi anemia group A protein

Gene ID: 2175

NCBI Accession: NP_000126

UniProt: O15360

Pathways: DNA Damage Repair