FANCG antibody (N-Term)

Catalog No. ABIN401267

Product Details anti-FANCG Antibody

Target: FANCG (Fanconi Anemia Complementation Group G (FANCG))

Binding Specificity: AA 1-12, N-Term

Reactivity: Human, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FANCG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against FANCG protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee (100 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 1-12 of human FANCG protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000-1/50,000. Western blot: 1/500-1/2,000 (Expect a band approximately 69 kDa in size corresponding toFANCG in the appropriate human tissue). Immunohistochemistry on Paraffin Sections: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.93 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for FANCG

Target: FANCG (Fanconi Anemia Complementation Group G (FANCG))

Alternative Name: XRCC9 / FANCG (FANCG Products)

Synonyms: xFANCG antibody, FAG antibody, XRCC9 antibody, AU041407 antibody, Xrcc9 antibody, Fanconi anemia complementation group G S homeolog antibody, Fanconi anemia complementation group G antibody, Fanconi anemia, complementation group G antibody, fancg.S antibody, FANCG antibody, Fancg antibody

Background: FANCG (also called Protein FACG or DNA-repair protein XRCC9) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCG may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. This protein may also function as a tumor suppressor gene. FANCG belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCG contains a 5-prime GC-rich untranslated region characteristic of housekeeping genes. The putative 622-amino acid protein has a leucine-zipper motif at its N-terminus. FANCG is mainly found within the nucleus although some protein is localized in the cytoplasm. This protein is highly expressed in testis and thymus and is also found in lymphoblasts. Fanconi anemia is an autosomal recessive disorder with diverse clinical symptoms, including developmental anomalies, bone marrow failure, and early occurrence of malignancies caused by defects in the FANCG gene. At the cellular level it is associated with hypersensitivity to DNAdamaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.Synonyms: DNA repair protein XRCC9, FACG, Fanconi anemia group G protein

Molecular Weight: 68.6 kDa

Gene ID: 2189

NCBI Accession: NP_004620

UniProt: O15287

Pathways: DNA Damage Repair