ROBO1 antibody (AA 1632-1644)

Catalog No. ABIN401279

Product Details anti-ROBO1 Antibody

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Binding Specificity: AA 1632-1644

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROBO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects ROBO-1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine (100 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide corresponding to amino acids 1632-1644 of Human ROBO-1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/30,000-1/160,000. Western blot: 1/500-1/3,000 (Expect a band at approx. 181 kDa in size corresponding to ROBO-1). Immunohistochemistry on Paraffin Sections: 2-10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: SStore the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for ROBO1

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Alternative Name: ROBO1 (ROBO1 Products)

Synonyms: DUTT1 antibody, SAX3 antibody, AW494633 antibody, AW742721 antibody, Gm310 antibody, ROBO1 antibody, dutt1 antibody, roundabout antibody, sax3 antibody, MGC122264 antibody, roundabout-1 antibody, roundabout guidance receptor 1 antibody, roundabout, axon guidance receptor, homolog 1 (Drosophila) antibody, roundabout guidance receptor 1 L homeolog antibody, ROBO1 antibody, Robo1 antibody, robo1 antibody, robo1.L antibody

Background: ROBO-1 (also called Roundabout homolog 1 precursor and Deleted in U twenty twenty (DUTT)) functions as a receptor for SLIT1 and SLIT2. The SLIT proteins are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. ROBO-1 may also be required for lung development. ROBO-1 is a type I membrane protein. ROBO-1 is a widely expressed protein with the exception of the kidney. Defects in ROBO1 may be a cause of breast and lung cancer. ROBO-1 maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line. Multiple splice variants have been identified for this protein.Synonyms: DUTT1, Deleted in U Twenty Twenty, ROBO-1, Roundabout homolog 1

Gene ID: 6091

NCBI Accession: NP_002932

UniProt: Q9Y6N7

Pathways: Positive Regulation of Endopeptidase Activity