EGR1 antibody (AA 94-108)

Catalog No. ABIN401280

Product Details anti-EGR1 Antibody

Target: EGR1 (Early Growth Response 1 (EGR1))

Binding Specificity: AA 94-108

Reactivity: Human, Mouse, Rat, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This affinity purified antibody is directed against EGR-1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee (100 %).Mouse and rat (86 %).
Species reactivity (tested):Human.

Purification: Immunoaffinty chromatography

Immunogen: Synthetic peptide corresponding to amino acids 94-108 of Human EGR-1

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 4,000 - 1: 16,000. Western blot: 1: 500 - 1: 3,000. Immunohistochemistry on paraffin sections: 2 μg/mL to 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.46 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for EGR1

Target: EGR1 (Early Growth Response 1 (EGR1))

Alternative Name: EGR1 (EGR1 Products)

Synonyms: AT225 antibody, G0S30 antibody, KROX-24 antibody, NGFI-A antibody, TIS8 antibody, ZIF-268 antibody, ZNF225 antibody, A530045N19Rik antibody, ETR103 antibody, Egr-1 antibody, Krox-1 antibody, Krox-24 antibody, Krox24 antibody, NGF1-A antibody, NGFIA antibody, Zenk antibody, Zfp-6 antibody, Zif268 antibody, egr antibody, Ngf1 antibody, Ngfi antibody, zif-268 antibody, EGR1 antibody, EGR-1-A antibody, Xegr-1 antibody, at225 antibody, egr-1 antibody, egr1 antibody, g0s30 antibody, krox-24 antibody, ngfi-a antibody, tis8 antibody, znf225 antibody, krox24 antibody, wu:fj64b05 antibody, wu:fq25f01 antibody, EGR-1 antibody, zenk antibody, EGR-1-B antibody, egr1-a antibody, egr1-b antibody, early growth response 1 antibody, early growth response protein 1 (egr-1) antibody, early growth response 1 L homeolog antibody, early growth response 1 S homeolog antibody, EGR1 antibody, CNK00890 antibody, Egr1 antibody, egr1.L antibody, egr1 antibody, egr1.S antibody

Background: EGR-1 (also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factorinduced protein A or NGFI-A, Transcription factor ETR103, and Zinc finger protein 225 or AT225) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site). EGR-1 activates the transcription of target genes whose products are required for mitogenesis and differentiation. EGR-1 is a nuclear protein induced by growth factors. Expression has been identified in a variety of cancers.Synonyms: AT225, EGR-1, ETR103, Early growth response protein 1, KROX-24, NGFI-A, Nerve growth factor-induced protein A, Protein Krox-24, Transcription factor ETR103, Transcription factor Zif268, Zif-268, Zif268

Gene ID: 1958

NCBI Accession: NP_001955

UniProt: P18146

Pathways: Regulation of Carbohydrate Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity