GLI3 antibody (AA 41-57)
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- Target See all GLI3 Antibodies
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
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Binding Specificity
- AA 41-57
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Reactivity
- Human, Mouse, Rat, Dog, Chicken, Monkey, Chimpanzee, Xenopus laevis
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GLI3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody is directed against Gli-3 protein.
- Cross-Reactivity (Details)
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Species reactivity (expected):Chimpanzee, squirrel Monkey, Xenopus laevis, Chicken, Canine (100 %).Mouse (94 %).Rat (88 %).
Species reactivity (tested):Human. - Purification
- Immunoaffinity Chromatography.
- Immunogen
- Synthetic peptide corresponding to amino acids 41-57 of Human Gli-3 protein
- Isotype
- IgG
- Top Product
- Discover our top product GLI3 Primary Antibody
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- Application Notes
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ELISA: 1/6,000-1/30,000. Western blot: 1/500-1/2,000. Immunohistochemistry on Paraffin Sections: 0.5 mg/mL-5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.12 M NaCl, pH 7.2, 0.09 % (w/v) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
- Alternative Name
- GLI3 (GLI3 Products)
- Synonyms
- ACLS antibody, GCPS antibody, GLI3-190 antibody, GLI3FL antibody, PAP-A antibody, PAPA antibody, PAPA1 antibody, PAPB antibody, PHS antibody, PPDIV antibody, xgli3 antibody, AI854843 antibody, AU023367 antibody, Bph antibody, Pdn antibody, Xt antibody, add antibody, im:7142603 antibody, GLI3 antibody, GLI family zinc finger 3 antibody, GLI family zinc finger 3 L homeolog antibody, GLI-Kruppel family member GLI3 antibody, GLI3 antibody, Gli3 antibody, gli3.L antibody, gli3 antibody
- Background
- Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-polysyndactyly syndrome (GCPS), an autosomal dominant disorder-affecting limb and craniofacial development. Two isoforms of human Gli-3 have been reported. One is the fulllength protein at ~170-190 kDa and the other is a truncated isoform at ~80 kDa.Synonyms: GLI-3, Zinc finger protein GLI3
- Gene ID
- 2737
- NCBI Accession
- NP_000159
- UniProt
- P10071
- Pathways
- Hedgehog Signaling
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