DLL4 antibody (Internal Region)

Catalog No. ABIN401283

Product Details anti-DLL4 Antibody

Target: DLL4 (delta-Like 4 (DLL4))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLL4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This affinity purified antibody is directed against DELTA-4. Expect a band ~74 kDa in size corresponding to DELTA-4 by Western blotting in the appropriate cell lysate or extract.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Chimpanzee (91 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide corresponding to an internal region of Human DELTA-4

Isotype: IgG

Application Details

Application Notes: ELISA: 1/5,000-1/25,000. Western blot: 1/500-1/2,500. Immunohistochemistry on Paraffin Sections: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for DLL4

Target: DLL4 (delta-Like 4 (DLL4))

Alternative Name: delta-Like Protein 4 / DLL4 (DLL4 Products)

Synonyms: hdelta2 antibody, Delta4 antibody, si:ch211-244p18.5 antibody, zgc:136596 antibody, delta like canonical Notch ligand 4 antibody, delta-like 4 (Drosophila) antibody, DLL4 antibody, Dll4 antibody, dll4 antibody

Background: DELTA-4 (also called delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. DELTA-4 plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.Synonyms: Delta 4, Drosophila Delta homolog 4

Gene ID: 54567

NCBI Accession: NP_061947

UniProt: Q9NR61

Pathways: Notch Signaling