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GLI2 antibody (GLI Family Zinc Finger 2) (AA 40-60)

Details for Product anti-GLI2 Antibody No. ABIN401285, Supplier: Log in to see
Antigen
  • AW546128
  • GLI2/GLI4
  • GLI3
  • HPE9
  • THP1
  • THP2
Alternatives
anti-Mouse (Murine) GLI2 antibody for Western Blotting
Epitope
AA 40-60, AA 46-60, Isoform alpha
24
9
8
5
2
2
2
1
1
1
Reactivity
Chimpanzee, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
50
28
14
4
3
1
1
1
1
1
1
1
Host
Rabbit
42
15
8
1
Clonality
Polyclonal
Conjugate
This GLI2 antibody is un-conjugated
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
49
41
25
15
3
2
1
1
1
1
Supplier
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Immunogen Synthetic peptide corresponding to amino acids 46-60 of Human Gli-2 (isoform alpha).
Isotype IgG
Specificity This antibody is directed against Gli-2 protein. Expect reactivity with all isoforms of this protein.
Cross-Reactivity (Details) Species reactivity (expected):Chimpanzee (100 %), Mouse and Rat (93 %), Bovine (86 %).
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography.
Alternative Name GLI2 (GLI2 Antibody Abstract)
Background Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is a nuclear protein thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes, Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. Multiple splice variants have been reported for this protein.Synonyms: GLI-2, THP, Tax helper protein, Zinc finger protein GLI2
Gene ID 2736
NCBI Accession NP_005261
UniProt P10070
Pathways Hedgehog Signaling
Application Notes ELISA: 1/2,000-1/12,000. Western blot: 1/500-1/2,000. Detection of Gli-2 may be enhanced if nuclear extracts areused instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced,making it difficult to detect in whole tissue homogenates. Immunohistochemistry on Paraffin Sections: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.1 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-GLI2 antibody (GLI Family Zinc Finger 2) (AA 40-60) (ABIN401285) anti-GLI Family Zinc Finger 2 (GLI2) (AA 40-60), (AA 46-60), (Isoform alpha) antibody
 image for anti-GLI2 antibody (GLI Family Zinc Finger 2) (AA 40-60) (ABIN401285) anti-GLI Family Zinc Finger 2 (GLI2) (AA 40-60), (AA 46-60), (Isoform alpha) antibody (Image 2)
Background publications Roessler, Ermilov, Grange, Wang, Grachtchouk, Dlugosz, Muenke: "A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2." in: Human molecular genetics, Vol. 14, Issue 15, pp. 2181-8, 2005

Regl, Kasper, Schnidar, Eichberger, Neill, Ikram, Quinn, Philpott, Frischauf, Aberger: "The zinc-finger transcription factor GLI2 antagonizes contact inhibition and differentiation of human epidermal cells." in: Oncogene, Vol. 23, Issue 6, pp. 1263-74, 2004

Ikram, Neill, Regl, Eichberger, Frischauf, Aberger, Quinn, Philpott: "GLI2 is expressed in normal human epidermis and BCC and induces GLI1 expression by binding to its promoter." in: The Journal of investigative dermatology, Vol. 122, Issue 6, pp. 1503-9, 2004

Tanimura, Teshima, Fujisawa, Yoshida: "A new regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1 and cloning of cDNAs encoding its binding proteins." in: Journal of virology, Vol. 67, Issue 9, pp. 5375-82, 1993

Ruppert, Kinzler, Wong, Bigner, Kao, Law, Seuanez, OBrien, Vogelstein: "The GLI-Kruppel family of human genes." in: Molecular and cellular biology, Vol. 8, Issue 8, pp. 3104-13, 1989

Kinzler, Ruppert, Bigner, Vogelstein: "The GLI gene is a member of the Kruppel family of zinc finger proteins." in: Nature, Vol. 332, Issue 6162, pp. 371-4, 1988