GGA1 antibody (AA 336-352)

Catalog No. ABIN401291

Product Details anti-GGA1 Antibody

Target: GGA1 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1))

Binding Specificity: AA 336-352

Reactivity: Human, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GGA1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against GGA1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 336-352 of Human GGA-1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000 - 1/50,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.35 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for GGA1

Target: GGA1 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1))

Alternative Name: GGA1 (GGA1 Products)

Synonyms: 4930406E12Rik antibody, AU016030 antibody, AW209092 antibody, fb33h10 antibody, im:6912200 antibody, wu:fb33h10 antibody, zgc:101044 antibody, putative golgi associated, gamma adaptin ear containing,arf binding protein 1 (gga1) antibody, golgi associated, gamma adaptin ear containing, ARF binding protein 1 antibody, golgi-associated, gamma adaptin ear containing, ARF binding protein 1 antibody, Smp_164030 antibody, Gga1 antibody, gga1 antibody, GGA1 antibody

Background: GGA1 (also known as ADP-ribosylation factor binding protein, Golgi-localized, gamma earcontaining, ARF-binding protein 1, Gamma-adaptin related protein 1) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with NECAP1, GGA2 and GGA3 as well as RABEP1, RABGEF1 and type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CDMPR, IGF2R/CI-MPR and BACE1. GGA1 is a ubiquitously membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.Synonyms: ADP-ribosylation factor-binding protein GGA1, Gamma-adaptin-related protein 1, Golgi-localized gamma ear-containing ARF-binding protein 1

Gene ID: 26088

NCBI Accession: NP_001001560

UniProt: Q9UJY5