VDAC1 antibody (AA 185-197)

Catalog No. ABIN401311

Product Details anti-VDAC1 Antibody

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Binding Specificity: AA 185-197

Reactivity: Human, Avian, Mammalian, Reptile

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VDAC1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (expected):Avian, Mammalia, Reptilia (100 %).
Species reactivity (tested):Human.

Purification: Immunoffinity Chromatography

Immunogen: Synthetic peptide corresponding to amino acids 185-197 of Human VDAC1/Porin1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000 - 1/40,000. Western blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.69 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody at-20 °C. This product is stable for several weeks at 2-8 °C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Alternative Name: VDAC / Porin (VDAC1 Products)

Synonyms: vdac1 antibody, VDAC1 antibody, 5076 antibody, ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1 antibody, ATVDAC1 antibody, T22N4.9 antibody, T22N4_9 antibody, voltage dependent anion channel 1 antibody, PORIN antibody, VDAC-1 antibody, AL033343 antibody, Vdac5 antibody, fa13f11 antibody, wu:fa13f11 antibody, zgc:85830 antibody, voltage-dependent anion channel 1 S homeolog antibody, voltage-dependent anion channel 1 antibody, voltage dependent anion channel 1 antibody, vdac1.S antibody, vdac1 antibody, VDAC1 antibody, Vdac1 antibody

Background: VDAC (also known as Voltage-dependent anionselective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL) is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger - see below). The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations.Synonyms: Outer mitochondrial membrane, Plasmalemmal porin, Porin 31HL, VDAC1, VDAC2, VDAC3, Voltage-dependent anionselective, channel protein 1, protein porin 1