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SMAD3 antibody (SMAD, Mothers Against DPP Homolog 3) (AA 417-425)

Details for Product anti-SMAD3 Antibody No. ABIN401333, Supplier: Log in to see
Antigen
  • madh3
  • madh3a
  • smad3
  • wu:fa99e03
  • HSPC193
  • HsT17436
  • JV15-2
  • LDS1C
  • LDS3
  • MADH3
  • XSmad3
  • XenMLP
  • Xmad3
  • madh3-A
  • Madh3
  • AU022421
  • Smad 3
  • mad3
Alternatives
anti-Human SMAD3 antibody for Western Blotting
Epitope
AA 417-425, C-Term
46
46
45
35
28
28
17
16
16
15
12
11
10
9
8
8
7
7
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
426
196
179
21
17
12
10
10
9
6
6
4
3
2
1
1
1
Host
Rabbit
390
47
Clonality
Polyclonal
Conjugate
This SMAD3 antibody is un-conjugated
18
17
15
8
8
8
6
4
4
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
308
209
196
77
66
48
31
19
18
16
8
7
2
2
1
Supplier
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Immunogen Synthetic peptide corresponding to amino acids 417-425 of Human SMAD3 protein
Isotype IgG
Specificity This antibody is directed against Smad3 protein. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated Human Smad3.
Cross-Reactivity (Details) Species reactivity (expected):Xenopus laevis, Xenopus tropicalis, Zebrafish, Rat, Mouse, Porcine, Bovine, Chicken.
Species reactivity (tested):Human.
Purification Affinity Chromatography
Alternative Name SMAD3 (SMAD3 Antibody Abstract)
Background Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (RSmads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-β pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-beta-mediated response.Synonyms: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3
Gene ID 4088
NCBI Accession NP_001138574
UniProt P84022
Research Area Signaling, Transcription Factors
Pathways Cell Division Cycle
Application Notes ELISA: 1/10,000 - 1/40,000. Western Blot: 1/500 - 1/3,000. Immunohistochemistry: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.17 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-SMAD3 antibody (SMAD, Mothers Against DPP Homolog 3) (AA 417-425) (ABIN401333) anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (C-Term) antibody
 image for anti-SMAD3 antibody (SMAD, Mothers Against DPP Homolog 3) (AA 417-425) (ABIN401333) anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (C-Term) antibody (Image 2)
Background publications Derynck, Zhang: "Smad-dependent and Smad-independent pathways in TGF-beta family signalling." in: Nature, Vol. 425, Issue 6958, pp. 577-84, 2003 (PubMed).

Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." in: Cell, Vol. 113, Issue 6, pp. 685-700, 2003 (PubMed).

Zhang, Feng, We et al.: "Receptor-associated Mad homologues synergize as effectors of the TGF-beta response." in: Nature, Vol. 383, Issue 6596, pp. 168-72, 1996 (PubMed).