MLF1IP antibody (Thr78)

Catalog No. ABIN401336

Product Details anti-MLF1IP Antibody

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Binding Specificity: Thr78

Reactivity: Human, Cow, Mouse, Dog, Rat, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLF1IP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against MLF1IP protein.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Bovine, Chimpanzee, Rat, Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500- 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.06 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Az

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for MLF1IP

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Alternative Name: MLF1IP (MLF1IP Products)

Synonyms: MGC147270 antibody, CENP50 antibody, CENPU antibody, CENPU50 antibody, KLIP1 antibody, PBIP1 antibody, CENP-U antibody, CENP-50 antibody, 1700029A22Rik antibody, centromere protein U antibody, MLF1 interacting protein antibody, cenpu antibody, MLF1IP antibody, CENPU antibody, Cenpu antibody

Background: Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1- dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.Synonyms: CENP-U(50), CENPU, Centromere protein U, ICEN24, Interphase centromere complex protein 24, KLIP1, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, PBIP, Polo-box-interacting protein 1

Gene ID: 79682

NCBI Accession: NP_078905

UniProt: Q71F23