Acetylated Lysine antibody

Antigen: Acetylated Lysine

Clonality: Polyclonal

Host: Rabbit

Reactivity: Bird (Avian)

Application: Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN401350

Additional Information

Immunogen: Prepared from whole rabbit serum produced by repeated immunizations with AcetylatedLysine conjugated KLH

Cross-Reactivity: Bird (Avian), Mammalian

Isotype: IgG  (Matching secondary antibodies)

Description: In the nucleus, DNA is tightly packed into nucleosomes generating an environment whichis highly repressive towards DNA processes such as transcription. Acetylation of lysineresidues within proteins has emerged as an important mechanism used by cells toovercome this repression. The acetylation of non-histone proteins such as transcriptionfactors, as well as histones appears to be involved in this process. Acetylation may resultin structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding ofacetylated peptides by the conserved bromodomain. Furthermore, recent findings suggestthat bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism inprotein-protein interactions in numerous cellular processes such as chromatin remodelingand transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role inthe regulation of many cellular processes including chromatin dynamics and transcription,gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNArepair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18deacetylases have been identified so far, but the mechanistic details of substrateselection and site specificity of these enzymes remain unclear. Over 40 transcriptionfactors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown tobe acetylated in vivo.

Specificity: This antibody reacts specifically with Acetylated Lysine residues. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG. Reactivity is specific for Acetylated Lysine. Species: Mammalian, Avian. Others not tested.

Application Details

Application Notes: ELISA: 1/2,500. Western Blot: 1/1,000. Immunofluorescence: 1/50. Immunoprecipitation: 10 µg/mg sample. Although not tested, this antibody is likely functional in RIA, Flow Cytometry andImmunohistochemistry.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Purification: Aff - Purified

Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing50% Glycerol without preservatives.

Storage: Store the antibody (in aliquots) at -20°C. Dilute only prior toimmediate use. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only