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IRAK2 antibody (Interleukin-1 Receptor-Associated Kinase 2) (C-Term)

Details for Product anti-IRAK2 Antibody No. ABIN401361, Supplier: Log in to see
Antigen
  • 6330415L08Rik
  • AI649099
  • IRAK-2
Alternatives
anti-Human IRAK2 antibody for Immunofluorescence
Epitope
C-Term
72
20
15
14
9
8
7
6
5
5
5
3
3
2
2
2
2
1
1
Reactivity
Human
185
42
22
Host
Rabbit
178
28
Clonality
Polyclonal
Conjugate
This IRAK2 antibody is un-conjugated
10
10
7
6
6
6
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
180
113
39
33
25
17
11
8
5
4
1
Supplier
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Immunogen Synthetic peptide corresponding to a region near the carboxy terminus of human IRAK-2 protein
Isotype IgG
Specificity This antibody is directed against IRAK-2 protein.
Purification Affinity Purified
Alternative Name IRAK2 (IRAK2 Antibody Abstract)
Background IRAK-2 (Interleukin-1 receptorassociated kinase 2). The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPSinduced NF-kappaB activation (2,3). Members of the IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.Synonyms: IRAK-2, Interleukin-1 receptor-associated kinase-like 2
Gene ID 3656
NCBI Accession NP_001561
UniProt O43187
Research Area Immunology, Innate Immunity, Cytokines, Receptors, Inflammation
Pathways TLR Signaling
Application Notes ELISA: 1/5000 - 1/20000. Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/300 - 1/600.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Background publications Zhang, Kirschning, Mancinelli, Xu, Jin, Faure, Mantovani, Rothe, Muzio, Arditi: "Bacterial lipopolysaccharide activates nuclear factor-kappaB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes." in: The Journal of biological chemistry, Vol. 274, Issue 12, pp. 7611-4, 1999

Yang, Mark, Gurney, Godowski: "Signaling events induced by lipopolysaccharide-activated toll-like receptor 2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 2, pp. 639-43, 1999

Muzio, Ni, Feng, Dixit: "IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1612-5, 1997