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RNF25 antibody (C-Term)

RNF25 Reactivity: Human, Mouse WB, IP, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN401373
  • Target See all RNF25 Antibodies
    RNF25 (Ring Finger Protein 25 (RNF25))
    Binding Specificity
    • 15
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 29
    • 17
    • 17
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 25
    • 4
    Rabbit
    Clonality
    • 27
    • 2
    Polyclonal
    Conjugate
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RNF25 antibody is un-conjugated
    Application
    • 29
    • 13
    • 7
    • 5
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
    Specificity
    This antibody is directed against RNF25 protein.
    Purification
    Affinity chromatography
    Immunogen
    Synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein
    Isotype
    IgG
    Top Product
    Discover our top product RNF25 Primary Antibody
  • Application Notes
    ELISA: 1/10,000 - 1/40,000. Western Blot: 1/200 - 1/2,000. Immunoprecipitation: 1 μg.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
  • Target
    RNF25 (Ring Finger Protein 25 (RNF25))
    Alternative Name
    RNF25 (RNF25 Products)
    Synonyms
    AO7 antibody, 0610009H16Rik antibody, ring finger protein 25 antibody, RNF25 antibody, Rnf25 antibody
    Background
    RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kappaB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).Synonyms: E3 ubiquitin-protein ligase RNF25, RING finger protein 25
    Gene ID
    64320
    NCBI Accession
    NP_071898
    UniProt
    Q96BH1
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