AHA1, Activator of Heat Shock 90kDa Protein ATPase Homolog 1 (Yeast) (AHSA1) antibody

Antigen: AHA1, Activator of Heat Shock 90kDa Protein ATPase Homolog 1 (Yeast) (AHSA1)

Synonyms: ahsa1, MGC52884, MGC132147, MGC80312, AHSA1, MGC89589, MGC136821, wu:fb83b05, zgc:136821

Clonality: Polyclonal

Host: Rabbit

Reactivity: Chimpanzee

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN401377

Additional Information

Characteristics: Synonyms: C14orf3, HSPC322, Activator of 90 kDa heat shock protein ATPase homolog 1

Alternative name: AHA1 / AHSA1

Swiss-Prot: O95433

Immunogen: Synthetic peptide corresponding to an internal region of human AHA1 protein

Cross-Reactivity: Chimpanzee, Human

Isotype: IgG  (Matching secondary antibodies)

Description: Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which isessential for its chaperone activity in vivo. The activation and/or stability of many of thekey regulatory and signaling proteins of the eukaryotic cell depend on their interaction withthe Hsp90 molecular chaperone. Hsp90 is assisted and regulated by co-chaperones thatparticipate in an ordered series both to assist clientprotein recruitment or release and tomodulate progress through the ATPase coupled chaperone cycle. Structural analysis andmutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes aconformational switch in the middle-segment catalytic loop (aa 370–390) of Hsp90 thatexposes the catalytic Arg380 and enables its interaction with ATP in the N-terminalnucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulatesHsp90-dependent stability of the folding of the cystic fibrosis transmembraneconductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1rescues misfolding of CFTR in cystic fibrosis.

Specificity: This antibody is directed against AHA1 protein. Species Reactivity: Tested: Human. Expected from sequence similarity: Chimpanzee.

Application Details

Application Notes: ELISA: 1/35,000 - 1/185,000. Western Blot: 1/500 - 1/3,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.08 mg/ml (by UV absorbance at 280 nm)

Purification: Aff - Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only