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ROCK2 antibody (pTyr256)

ROCK2 Reactivity: Human, Mouse, Rat, Cow, Dog, Chicken, Drosophila melanogaster, Xenopus laevis WB, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN401393
  • Target See all ROCK2 Antibodies
    ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))
    Binding Specificity
    • 16
    • 15
    • 9
    • 8
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr256
    Reactivity
    • 105
    • 67
    • 67
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Dog, Chicken, Drosophila melanogaster, Xenopus laevis
    Host
    • 99
    • 7
    • 1
    Rabbit
    Clonality
    • 96
    • 11
    Polyclonal
    Conjugate
    • 48
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This ROCK2 antibody is un-conjugated
    Application
    • 75
    • 36
    • 27
    • 27
    • 18
    • 17
    • 17
    • 11
    • 9
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificity
    This phospho-specific polyclonal antibody reacts with Human ROCK-2 pTyr256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Expect a band approximately 164 kDa in size corresponding to phosphorylated ROCK-2
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse, Rat, Chicken, Canine, Bovine, Xenopus, Drosophila.
    Species reactivity (tested):Human.
    Purification
    Affinity Chromatography.
    Immunogen
    Synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2
    Isotype
    IgG
    Top Product
    Discover our top product ROCK2 Primary Antibody
  • Application Notes
    ELISA: 1/2,000-1/10,000. Western Blot: 1/200-1/2,000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.1 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-28 °C for longer.
  • Target
    ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))
    Alternative Name
    ROCK2 (ROCK2 Products)
    Synonyms
    rocka-a antibody, xROK antibody, rock2 antibody, rok2 antibody, ROCK2 antibody, B230113H15Rik antibody, ROKalpha antibody, Rho-kinase antibody, Rock-II antibody, Rock2m antibody, mKIAA0619 antibody, ROCK-II antibody, ROK antibody, Rho-associated, coiled-coil containing protein kinase 2 L homeolog antibody, rho-associated, coiled-coil containing protein kinase 2a antibody, Rho associated coiled-coil containing protein kinase 2 antibody, Rho-associated, coiled-coil containing protein kinase 2 antibody, Rho-associated coiled-coil containing protein kinase 2 antibody, rock2.L antibody, rock2a antibody, ROCK2 antibody, Rock2 antibody
    Background
    Progression of tumors to invasive and metastatic forms requires that tumor cells undergo dramatic morphological changes, a process regulated by Rho GTPases. Increased levels of RhoA or Rh °C, as well as the Rho effector proteins ROCK-1 or ROCK-2, were observed in various human carcinomas. In vivo studies have also shown that ROCK inhibition reduced the invasiveness of several tumor cell lines. It is suggested that Rho and ROCK signaling contribute to the morphological changes and metastatic behavior of some tumor cells. Thus, elevated Rho and ROCK expression is associated with tumorigenesis, particularly during the progression to invasive and metastatic phenotypes. Phosphorylation of certain residues in ROCK-2 may be critical for its kinase activity.Synonyms: KIAA0619, ROCK-2, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, coiled-coil-containing protein kinase 2, p164ROCK-2
    Gene ID
    9475
    NCBI Accession
    NP_004841
    UniProt
    O75116
    Pathways
    Microtubule Dynamics, WNT Signaling, Tube Formation
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