Ataxia Telangiectasia and Rad3 Related (ATR) antibody

Antigen: Ataxia Telangiectasia and Rad3 Related (ATR)

Synonyms: FRP1, MEC1, SCKL, SCKL1, ATR, atr, XATR, frp1, mec1, sckl, sckl1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine)

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN401402

Additional Information

Characteristics: Synonyms: FRAP-related protein 1, Serine/threonine-protein kinase ATR, Ataxia telangiectasia andRad3-related protein

Alternative name: ATR / FRP1

Swiss-Prot: Q13535

Immunogen: Synthetic peptide corresponding to an internal region of human ATR protein

Cross-Reactivity: Dog (Canine), Fish, Human, Monkey, Mouse (Murine), Rat (Rattus), Xenopus laevis

Isotype: IgG  (Matching secondary antibodies)

Description: Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is aphosphatidylinositol kinase (PK)-related kinase which functions in response to DNAdamage and repair as well as at DNA replication checkpoints during the cell cycle. ATRactivates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR),ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNAPK forwhich DNA stimulates the observed kinase activity. Chromosomal remodeling proteinshave also been reported to associate with ATR complexes, including histone deacetylases(HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17,RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis andpromote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can alsobe found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin duringSphase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replicationfork stalling.

Specificity: This antibody is specific for ATR protein. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat, Monkey, Canine, Fish, Xenopus.

Application Details

Application Notes: ELISA: 1/15,000 - 1/70,000. Western Blot: 1/1,000 - 1/5,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.28 mg/ml (by UV absorbance at 280 nm)

Purification: Aff - Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only