NCOA3 antibody (Internal Region, Isoform A)

Catalog No. ABIN401408

Product Details anti-NCOA3 Antibody

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Binding Specificity: Internal Region, Isoform A

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOA3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts with several isoforms of NCOA3 protein (a, b, d, e and f).

Cross-Reactivity (Details): Species reactivity (expected):Marcaque, Canine, Bovine, Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/175000. Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.24 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Alternative Name: NCOA3 (NCOA3 Products)

Synonyms: NCOA3 antibody, ACTR antibody, AIB-1 antibody, AIB1 antibody, CAGH16 antibody, CTG26 antibody, KAT13B antibody, RAC3 antibody, SRC-3 antibody, SRC3 antibody, TNRC14 antibody, TNRC16 antibody, TRAM-1 antibody, bHLHe42 antibody, pCIP antibody, 2010305B15Rik antibody, AW321064 antibody, Actr antibody, Aib1 antibody, Rac3 antibody, Src3 antibody, Tram-1 antibody, Tram1 antibody, p/Cip antibody, pCip antibody, actr antibody, aib-1 antibody, aib1 antibody, cagh16 antibody, ctg26 antibody, kat13b antibody, pcip antibody, rac3 antibody, src-3 antibody, src3 antibody, tnrc14 antibody, tnrc16 antibody, tram-1 antibody, xSRC-3 antibody, nuclear receptor coactivator 3 antibody, nuclear receptor coactivator 3 S homeolog antibody, NCOA3 antibody, Ncoa3 antibody, ncoa3.S antibody

Background: The NCOA3 (also known as nuclear receptor coactivator 3 isoform a) is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions in a hormone-dependent fashion. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. NCOA3 probably acts via remodeling of chromatin and is involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. This antibody was raised against variant 1 which is the longer isoform (a). In addition, a polymorphic repeat region is found in the C-terminus of the protein.Synonyms: ACTR, AIB-1, AIB1, Amplified in breast cancer 1 protein, BHLHE42, CBP-interacting protein, Class E basic helix-loop-helix protein 42, NCoA-3, Nuclear receptor coactivator 3, RAC-3, RAC3, Receptor-associated coactivator 3, SRC-3, Steroid receptor coactivator protein 3, TRAM-1, TRAM1, Thyroid hormone receptor activator molecule 1, pCIP

Gene ID: 8202

NCBI Accession: NP_001167559

UniProt: Q9Y6Q9

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha