ABCB5 antibody (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5) (Internal Region)

Details for Product anti-ABCB5 Antibody No. ABIN401433, Supplier: Log in to see
Antigen
  • MDR1
  • wu:fi81f06
  • mdr1
  • im:7158730
  • wu:fc18f02
  • ABCB5alpha
  • ABCB5beta
  • EST422562
  • 9230106F14Rik
  • RGD1566342
  • ABCB5
  • ATP-binding cassette, sub-family B (MDR/TAP), member 5
  • ATP-binding cassette sub-family B member 5
  • ATP-binding cassette, subfamily B (MDR/TAP), member 5
  • multidrug resistance protein 1-like
  • ABCB5
  • abcb5
  • TGME49_049820
  • PAAG_04387
  • VDBG_00160
  • VDBG_02292
  • Abcb5
  • LOC536724
Epitope
Internal Region
34
21
12
12
11
6
2
2
1
1
1
1
Reactivity
Human, Monkey
113
18
6
2
2
Host
Rabbit
60
28
23
2
Clonality
Polyclonal
Conjugate
This ABCB5 antibody is un-conjugated
4
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
73
57
48
44
41
39
37
4
2
1
1
1
1
Options
Supplier
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Immunogen Synthetic peptide corresponding to residues corresponding to an internal region of Human ABCB5
Isotype IgG
Specificity This antibody is specific for ABCB5 protein.
Cross-Reactivity (Details) Species reactivity (expected):Monkey (85 %), Rat (68 %), Mouse (62 %).
Species reactivity (tested):Human.
Purification Affinity Chromatography
Alternative Name ABCB5 (ABCB5 Antibody Abstract)
Background ATP-Binding Cassette proteins comprise a superfamily of transporters with a wide variety of substrates. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. It has been identified as a novel drug transporter in human malignant melanoma. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5.Synonyms: ATP-binding cassette sub-family B member 5
Gene ID 340273
NCBI Accession NP_001157413
UniProt Q2M3G0
Application Notes ELISA: 1/10,000-1/50,000. Western Blot: 1/20,000. Trial Size: ELISA: 1/10,000- 1/15,000. Western Blot: 1/10,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
 image for anti-ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5) (Internal Region) antibody (ABIN401433) anti-ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5) (Internal Region) antibody
Background publications Munday, Hiscox, Barr: "Quantitative proteomic analysis of A549 cells infected with human respiratory syncytial virus subgroup B using SILAC coupled to LC-MS/MS." in: Proteomics, Vol. 10, Issue 23, pp. 4320-34, 2010 (PubMed).

Chen, Szakács, Annereau, Rouzaud, Liang, Valencia, Nagineni, Hooks, Hearing, Gottesman: "Principal expression of two mRNA isoforms (ABCB 5alpha and ABCB 5beta ) of the ATP-binding cassette transporter gene ABCB 5 in melanoma cells and melanocytes." in: Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society, Vol. 18, Issue 2, pp. 102-12, 2005 (PubMed).

Frank, Margaryan, Huang, Schatton, Waaga-Gasser, Gasser, Sayegh, Sadee, Frank: "ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma." in: Cancer research, Vol. 65, Issue 10, pp. 4320-33, 2005 (PubMed).

Chen, Gottesman: "Useful tool to generate unidirectional deletion vectors by utilizing the star activity of BamHI in an NcoI-BamHI-XhoI cassette." in: BioTechniques, Vol. 38, Issue 2, pp. 198, 200, 202, 204, 2005 (PubMed).

Huang, Anderle, Bussey, Barbacioru, Shankavaram, Dai, Reinhold, Papp, Weinstein, Sadée: "Membrane transporters and channels: role of the transportome in cancer chemosensitivity and chemoresistance." in: Cancer research, Vol. 64, Issue 12, pp. 4294-301, 2004 (PubMed).

Frank, Pendse, Lapchak, Margaryan, Shlain, Doeing, Sayegh, Frank: "Regulation of progenitor cell fusion by ABCB5 P-glycoprotein, a novel human ATP-binding cassette transporter." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 47156-65, 2003 (PubMed).

Taipalensuu, Törnblom, Lindberg, Einarsson, Sjöqvist, Melhus, Garberg, Sjöström, Lundgren, Artursson: "Correlation of gene expression of ten drug efflux proteins of the ATP-binding cassette transporter family in normal human jejunum and in human intestinal epithelial Caco-2 cell monolayers." in: The Journal of pharmacology and experimental therapeutics, Vol. 299, Issue 1, pp. 164-70, 2001 (PubMed).

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