p130 antibody

Catalog No. ABIN401457

Product Details anti-p130 Antibody

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Reactivity: Human, Rat, Dog, Horse, Cow, Chimpanzee, Orang-Utan, Opossum

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p130 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This antiserum is directed against Spa310 and reacts with the Spa3120 domain of pRb2/p130 from human tissues.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Orangutan, Horse, CAnine, Bovine, Rat, Opossum.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein. A residue of cysteine was added to facilitate coupling

Isotype: IgG

Application Details

Application Notes: ELISA: 1/75,000 - 1/100,000. Western Blot: 1/500 - 1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.39 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for p130

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Alternative Name: RBL2 / p130 (RBL2 Products)

Synonyms: RBL2 antibody, RBR2 antibody, RRB2a antibody, PRB2 antibody, RBR-2 antibody, Rb2 antibody, p130 antibody, P130 antibody, retinoblastoma-like 2 (p130) antibody, RB transcriptional corepressor like 2 antibody, related to retinoblastoma 2 antibody, RBL2 antibody, LOC541652 antibody, Rbl2 antibody

Background: Spa310 is a 39 aa-long polypeptide encoded by a sequence which resides in the spacer region of the tumor suppressor Rb2 gene. Rb2 is a member of the retinoblastoma (Rb) gene family. Proteins in this family, which also include pRb/p105 and pRb/p107, are important cellular factors which play wellrecognized roles as tumor and growth suppressors. Both p107 and pRb2/p130 share the ability to physically interact and inhibit the kinase activity of the Cdk2/Cyclin A and Cdk2/Cyclin E complexes, which play critical roles in cell cycle regulation. Spa310 is the region of the pRb2/p130 protein that is responsible for Cdk2/Cyclin E/A inhibition. Spa310 has also been shown to suppress cell growth as observed by colony formation, and to reduce volume of tumor growth in nude mice, likely through arrest in the G 0 /G 1 phase of the cell cycle. Understandably, the Spa310 small molecule represents a potentially significant pharmaceutical product in the treatment of hyperproliferative disorders.Synonyms: 130 kDa retinoblastoma-associated protein, RB2, RBR-2, Retinoblastoma-like protein 2, Retinoblastoma-related protein 2, p130, pRb2

Gene ID: 5934

NCBI Accession: NP_005602

UniProt: Q08999

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases