SMAD, Mothers Against DPP Homolog 1 (SMAD1) antibody

Antigen: SMAD, Mothers Against DPP Homolog 1 (SMAD1)

Synonyms: BSP1, JV41, JV4-1, MADH1, MADR1, Madh1, bsp1, jv41, Xmad1, jv4-1, madh1, madr1, Xsmad1, smad-1, MGC89254, SMAD1, MGC138917

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine)

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN401472

Additional Information

Characteristics: Synonyms: SMAD family member 1, SMAD-1, SMAD 1, MADH1, MAD homolog 1, MADR1, Mad-relatedprotein 1, BSP1, BSP-1, JV4-1, Mothers against decapentaplegic homolog 1, Mothers againstDPP homolog 1, Transforming growth factor-beta-signaling protein 1

Alternative name: SMAD1

Swiss-Prot: Q15797

Immunogen: Synthetic peptide corresponding to the region of amino acids containing serine 206 ofhuman SMAD1 protein

Cross-Reactivity: Dog (Canine), Chimpanzee, Chicken, Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine), Bonobo, Cow (Bovine), Duckbill (Platypus),

Isotype: IgG  (Matching secondary antibodies)

Description: SMAD1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPPhomolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediatemultiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP(Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD orR-SMAD). BMPs are involved in a range of biological activities including cell growth,apoptosis, morphogenesis, development and immune responses. SMAD proteins havebeen implicated as downstream effectors of TGF beta/BMP signaling. In response to BMPligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206,are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenicgrowth factors, or by recombinant ERK in vitro, this can be tested by treating cells with EGFor in cancer cells where Ras is activated. The phosphorylated form of this protein forms acomplex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 andSMURF2, and undergoes ubiquitination and proteasomemediated degradation.

Specificity: This antibody is directed against SMAD1 protein. Species Reactivity: Tested: Human. Expected from sequence similarity: Duckbill platypus, Drosophila, Mouse, Opossum,

Application Details

Application Notes: ELISA: 1/25,000 - 1/30,000. Western Blot: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.31 mg/ml (by UV absorbance at 280 nm)

Purification: Aff - Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Storage Conditions for Trial Size: This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volumedilute 1: 10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mixthoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use thisintermediate dilution when calculating final dilutions as recommended below. Shelf life: 3 month from despatch.

Research Area: Signaling, Transcription Factors

Restrictions: For Research Use only