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Details for Product No. ABIN401485

V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region) antibody

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Antigen
Synonyms
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, pkb, xAct, akt-1, v-akt, v-akt1, Akt, AKT1, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, AKT/PKB, Akt/PKB, Akt1, CG4006, D-Akt,  ... show more
Epitope
»Alternatives Internal Region
Reactivity
»Alternatives Chicken, Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Sheep
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 7 references available
Catalog no. ABIN401485
Quantity 25 µL
Price
280.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide corresponding to an internal region of human AKT1 protein. A residue of cysteine was added to facilitate coupling
Isotype IgG
Specificity This antibody reacts to AKT.
Purification Affinity chromatography
Alternative Name AKT1 / PKB
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.
Alternate names: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431.1
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Application Notes ELISA: 1/5,000 - 1/20,000. Western Blot: 1/500 - 1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.9 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing Sodium Azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Background publications Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001 (PubMed).

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001 (PubMed).

Dan, Adli, Baldwin: "Regulation of mammalian target of rapamycin activity in PTEN-inactive prostate cancer cells by I kappa B kinase alpha." in: Cancer research, Vol. 67, Issue 13, pp. 6263-9, 2007 (PubMed).

Liu, Zhou, Qian et al.: "AKT1 amplification regulates cisplatin resistance in human lung cancer cells through the mammalian target of rapamycin/p70S6K1 pathway." in: Cancer research, Vol. 67, Issue 13, pp. 6325-32, 2007 (PubMed).

Chu, Li, Singh et al.: "BCR-tyrosine 177 plays an essential role in Ras and Akt activation and in human hematopoietic progenitor transformation in chronic myelogenous leukemia." in: Cancer research, Vol. 67, Issue 14, pp. 7045-53, 2007 (PubMed).

Pino, Alata, Dedonder et al.: "Photon induced isomerization in the first excited state of the 7-azaindole-(H2O)3 cluster." in: Physical chemistry chemical physics : PCCP, Vol. 13, Issue 13, pp. 6325-31, 2011 (PubMed).

Senthilkumar, Thirumurugan: "GSTM1 and GSTT1 Allele Frequencies among Various Indian and non-Indian Ethnic Groups." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 13, Issue 12, pp. 6263-7, 2013 (PubMed).

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)", type "Antibodies"
Hosts (655), (148), (8), (5), (4), (3)
Reactivities (793), (523), (501), (175), (154), (117), (95), (21), (19), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (590), (334), (205), (174), (143), (130), (117), (70), (31), (17), (17), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Conjugates (21), (15), (13), (13), (13), (13), (13), (13), (13), (13), (13), (9), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1)
Epitopes (125), (77), (44), (44), (43), (35), (30), (28), (27), (18), (16), (16), (9), (9), (7), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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